细胞表面蛋白分离试剂盒(Bacterial Surface Protein Isolation Kit) 产品及特点 本产品为方便的生物素化并分离细胞表面蛋白,可用于蛋白质免疫印迹分析。 它具有如下特点: 1. 分离细胞表面蛋白,降低全细胞蛋白的复杂性。 2. 高效回收标记的蛋白,使用可切除的生物素可得到接近100% 的细胞表面蛋白的回收率。
上海玉博生物科技有限公司 主要致力于“Cell Surface Protein Isolation Kit pierce-89881”的生产销售。多年的“Cell Surface Protein Isolation Kit pierce-89881”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用户信赖。
Protein Assays Measuring protein concentration Accurately quantifying total protein concentration is a key step in most experiments and workflows involving isolation, separation, and analysis of proteins. We offer a variety of assay reagents, kits, and standards for protein quantitation by fluorescent or ...
IP analyses were performed with the μMACS Epitope-Tagged Protein Isolation Kit (Miltenyi Biotec) according to the manufacture’s instruction with small modifications. The supernatants were incubated with μMACS anti-GFP microbeads for 2 h at 4 °C on a rotator and then applied to μMACS...
kit. Western blot analysis probed with NKCC1 and β-actin antibodies for both total lysate and membrane fractions. β-actin was used as the loading control and equal amounts of lysate protein were used for the isolation of cell membrane proteins (lysate protein concentration was measured by ...
中文名称: EXOSOME ISOLATION KIT (FROM PLASMA, FOR PROTEIN DETECTION)(血浆外泌体提取试剂盒,用于蛋白检测) 中文同义词: EXOSOME ISOLATION KIT (FROM PLASMA, FOR PROTEIN DETECTION)(血浆外泌体提取试剂盒,用于蛋白检测) 英文名称: EXOSOME ISOLATION KIT (FROM PLASMA, FOR PROTEIN DETECTION) 英文同义词: EXO...
This is not surprising, once neutrophils are isolated from blood, and although extreme care is taken during the isolation/incubation process, these immune cells already sense environment alterations that lead to cellular stress. Supplemental Table 1A shows all the differentially regulated proteins found ...
Supernatants were removed for SCFA analysis, and the resulting pellets were used for DNA isolation as described previously and analyzed according to the assays described below. All reagents and materials were degassed under anaerobic conditions for 24 h prior to use. Quantitative Microbial Protein ...
(Miltenyi Biotec 130-042-401) followed by enrichment of neuronal cells using the adult neuron isolation kit (Miltenyi Biotec 130-126-603) and LS columns. Enriched cell populations were fixed using Cytofix Fixation Buffer (BD 554655) at 4 °C for 30 min and blocked with 1:50 TruStain ...
μg/ml of spectinomycin (Spc). Plasmids were isolated using High Pure Plasmid Isolation Kit (Roche), checked by PCR and capillary sequencing, and used for engineering of the OSYRIS cells. The following sections outline the construction of the main plasmids....