Protein - Isolation, Determination, Structure: Animal material usually contains large amounts of protein and lipids and small amounts of carbohydrate; in plants, the bulk of the dry matter is usually carbohydrate. If it is necessary to determine the amou
method of isolation and recovery of protein hormones derived from pituitary tissuesProtein hormones are fractionated and isolated from extracts of pituitary glands or tissues. Increased yield and purity of the growth hormone is obtained by using controlled amounts of polyethylene glycol as precipitating ...
Protein turnover is critical to cellular physiology as well as to the growth and maintenance of tissues. The unique synthesis and degradation rates of each protein help to define tissue phenotype, and knowledge of tissue- and protein-specific half-lives
If recovery of high molecular weight DNA is desired, use a loosely fitting homogenizer in the initial homogenization step of the RNA Isolation Protocol. Avoid using a Polytron homogenizer. If the DNA is isolated only for quantitative purposes: a...
M-PEK Kit A convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. Extraction is based on association of proteins with cellular membranes rather than on their hydrophobicity. Resulting samples are suitable for use in funct...
SDS-PAGE Sample Prep Kit Introduction Historically, mechanical disruption has been used to lyse cells and tissues; however, detergent-based solutions have more recently been developed to efficiently lyse cells and enable the separation ...
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The secondary antibody was visualized by using a chemiluminescent reagent (Pierce ECL kit, Thermo Fisher Scientific, Waltham, MA, USA). Uncropped images of blots are shown in Supplementary Fig. 11. Isolation of TILs Freshly isolated primary tumor tissues (from two or three mice/group) were ...
Direct reversion of cancers into normal-like tissues is an ideal strategy for cancer treatment. Recent reports have showed that defined transcription factors can induce reprogramming of cancer cells into pluripotent stem cells, supporting this notion. Here, we have developed a reprogramming method that...
Serial sections of human embryos representing various stages of development were used for in vitro hybridization to explore the pattern of Frzb expression during embryonic development. Tissues from human embryos ranging from 6 to 13 weeks of gestation, estimated on the basis of crown-rump length and...