The Total Exosome RNA and Protein Isolation Kit enables isolation of total RNA and proteins from exosome samples extracted from biological fluids (e.g., by using Total Exosome Isolation (from cell culture media) or Total Exosome Isolation (from serum)).• Extracts highly pure total RNA (...
Isolation of serum protein organometallic corrosion products from 316LSS and HS-21 in vitro and in vivo. J Biomed Mater Res. 1984;18(1):99-114.Woodman JL, Black J, Jiminez SA. Isolation of serum protein organometallic corrosion products from 316LSS and HS-21 in vitro and in vivo. J ...
we purified each of these proteins in isolation. Each payload was tagged with an affinity and solubility tag (6×His–Strep–SUMO) and was transformed intoE. coliBL21 (DE3) competent cells (Sigma-Aldrich CMC0016). Colonies were inoculated into 5 ml TB medium and...
Mitochondria Isolation Kit for Tissue Pierce 89801 KIT 2597 RED Device Insert Removal Tool Pierce 89812 EACH 1337 Mem-PER Membrane Protein Extraction Reagent Pierce 89826 KIT 2418 Mitochondria Isolation Kit Pierce 89874 KIT 2406 Chemilumi-Nucleic Acid Detection Kit/化学发光核酸检测试剂盒 Pierce 89880 ...
Dynabeads Protein G beads allow for the isolation of most mammalian immunoglobulins (Ig). The amount of Ig captured depends on the concentration of Ig in the starting sample and on the type and source of the Ig. 100 μL of Dynabeads Protein G will isolate approximately 25–30 μg human Ig...
Single cell isolation The single cells were incubated on ice with a viability dye (LIVE/DEAD Viability Kit, Molecular Probes, L3224) for approximately 15 min and then passed through a 40 μM filter before sorting. Cells were sorted on a BD FACS ARIAIII into a 96-well plate. We used...
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. - Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding ant...
Supernatants were removed for SCFA analysis, and the resulting pellets were used for DNA isolation as described previously and analyzed according to the assays described below. All reagents and materials were degassed under anaerobic conditions for 24 h prior to use. Quantitative Microbial Protein ...
electrophoresis was performed on a 2% agarose gel for DNA detection. The PCR products were mixed in equal proportions, and then Qiagen Gel Extraction Kit (Qiagen, Germany) was used to purify the mixed PCR products. Following the manufacturer’s recommendations (Novogene Co., Ltd., Tianjin, ...
Milk enriched with functional ingredients of milk proteins delivers health and nutritional benefits, and it can be particularly recommended to consumers with increased protein requirements. The aim of this study was to evaluate the applicability of casein and serum protein preparations obtained by membrane...