Proteins are the essential biological macromolecules required to perform nearly all biological processes, and cellular functions. Proteins rarely carry out their tasks in isolation but interact with other proteins (known as protein–protein interaction)
Molecules that covalently engage target proteins are widely used as activity-based probes and covalent drugs. The performance of these covalent inhibitors is, however, often compromised by the paradox of efficacy and risk, which demands a balance between
Software defaults were used to control the false discovery rate (FDR) and only peptides spectra with less than 1% FDR and less than 30% isolation interference were included in analysis. Protein log 2 relative abundances were estimated using the method of Herbrich et al. [11]. In this ...
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Supernatants were removed for SCFA analysis, and the resulting pellets were used for DNA isolation as described previously and analyzed according to the assays described below. All reagents and materials were degassed under anaerobic conditions for 24 h prior to use. Quantitative Microbial Protein ...
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2. Materials and Methods 2.1. Cell Culture. The human lens epithelial cell line HLE-B3 was obtained from laboratories of the Tianjin Medical Uni- 2 BioMed Research International versity Eye Hospital (Tianjin, China) and incubated in Dul- becco's Modified Eagle Medium (DMEM) with 10% fetal ...
1. Such interactions vary from being permanent to transient2,3. Some protein–protein interactions are specific for a pair of proteins, while some proteins are promiscuous and interact with many partners. This complexity of interactions is a challenge both for experimental and computational methods....
Protein arginine methylation: cellular functions and methods of analysis. Biochim. Biophys. Acta 1764, 1890–1903 (2006). Article CAS PubMed Google Scholar Chuikov, S. et al. Regulation of p53 activity through lysine methylation. Nature 432, 353–360 (2004). Article CAS PubMed Google Scholar...
12862_2010_1693_MOESM1_ESM.PDF Additional file 1: Promoter regions. The structure of NmeGp1 promoter regions from sponges S. domuncula and A. queenslandica. The most plausible putative binding sites for transcription factors identified by TFSEARCH are marked. Motifs shared with human Nme1 promoter...