Isolation of Protein and Peptides from Symbiont Bacteria of Green Algae, Caulerpa lentillifera and Their Potency as Anticancerdoi:10.31557/APJCP.2025.26.3.767Arfah, Rugaiyah AndiAhmad, AhyarKhairunnur, SitiTaba, PaulinaIrfandi, RizalKarim, HarningsihAsian Pacific Journal...
The Hib bacteria are a single serotype (b) with several licensed Hib conjugate vaccines (HibCV)196 with different carrier proteins (TT, diphtheria toxoid, CRM197, and an outer membrane protein complex from N. meningitidis) of different sizes, distinct polysaccharide chain lengths and distinct conju...
Protocol for purification of inclusion bodies & protein refolding at Profacgen Step 1. Preparation of inclusion bodies: a. Harvest bacteria after induction. b. Lyse bacteria by sonication in the buffer containing Tri-HCl, NaCl, EDTA, NaN3, Triton-X100, PMSF and DTT. 50 ml aliquot usually wor...
Second, there is no need to separate free RNAs on urea–PAGE, which is a necessary step to separate linker−linker ligation from previous CLIP protocol. Third, the usage of high concentration of RNA linkers can be avoided, which alleviates the aforementioned self-ligation problem as well. In...
Adsorption to the cell surface is the first step in the phage life cycle. Prevention of adsorption through modification of surface receptors serving as phage entry points is the first line of defence for bacteria, and it is a prevalent target for phage resistance mechanisms11. Bacteria thwart pha...
However, many membrane proteins do not form a functional complex in a heterologous system, and few methods exist to purify sufficient protein from a native source for use in biochemical, biophysical and structural studies. Here, we provide a detailed protocol for the isolation of membrane protein ...
Proteins can come from many sources, including the following: native sources such as mammalian cell cultures, tissues or bodily fluids; overexpression in a model system such as bacteria, yeast, insect or mammalian cells...
Ngo JT, Schuman EM, Tirrell DA (2013) Mutant methionyl-tRNA synthetase from bacteria enables site-selective N-terminal labeling of proteins expressed in mammalian cells. Proc Natl Acad Sci USA 110(13):4992–4997. https://doi.org/10.1073/pnas.1216375110 Article PubMed PubMed Central Google Scho...
Thus, TrypN digestion was performed according to the phase-transfer surfactants (PTS) protocol (29) in this study. Next, keeping in mind the need to separate protein N-terminal–derived peptides with both His residues and unmodified N termini from TrypN-digested internal peptides, we ...
Label-free protein characterization at surfaces is commonly achieved using digestion and/or matrix application prior to mass spectrometry. We report the assignment of undigested proteins at surfaces in situ using secondary ion mass spectrometry (SIMS). B