Careful consideration of an appropriate method is required to avoid incorrect or biased data that may lead to misinterpretations.In this chapter, we will provide a broad overview of the current state of the art in matters of single-cell isolation methods mostly applied for sequencing-based ...
[Problem] To provide a single-cell isolation method for Botryococcus braunii whereby it is possible to increase the range of reagents that can be used to isolate single cells from the extracellular matrix of Botryococcus braunii. [Solution] Single cells are isolated from the extracellular matrix ...
We report a flexible single-cell isolation method by imaging-assisted hydrogel formation. Our approach consists of imaging-aided selective capture of cells of interest by encasing them into a polymeric hydrogel, followed by removal of unwanted cells and
The isolation of single bacterial cells anthracis. (3) The third reason advanced for the presentation of this note is in the nature of a plea for the more extended use of the single cell isolation method as a routine measure. The saving of labour and of time involved... JH Mason,PJD To...
Quantifying heterogeneities within cell populations is important for many fields including cancer research and neurobiology; however, techniques to isolate individual cells are limited. Here, we describe a high-throughput, non-disruptive, and cost-effective isolation method that is capable of capturing ind...
1). Isolation of single cells mainly includes cell selection, random seeding/dilution, laser microdissection (LCM), fluorescence-activated cell sorting (FACS), and microfluidic/microplate methodology [35, 36]. FACS is the most commonly used method. Manual cell selection is used during the early ...
the genome-wide profiling of chromatin accessibility can be used to identify candidate regulatory genomic regions in a tissue or cell type. Multiple biochemical methods have been developed to profile chromatin accessibility, both in bulk and at the single-cell level. Depending on the method, enzymati...
cell-level downstream analysis -细胞水平的下游分析 gene-level downstream analysis -基因水平的下游分析 1. Pre-processing and visualization experimental workflow single-cell dissociation, single-cell isolation, library construction, and sequencing 分别为以上四步走 ...
A rapid and efficient single-cell manipulation method for screening antigen-specific antibody–secreting cells from human peripheral blood[J]. Nature Medicine, 2009, 15(9):1088-1092. [9] Fitzgerald V., Leonard P., et al. Single cell screening approaches for antibody discovery[J]. Methods, ...
This method infers the potential interaction strength between two cell subsets based on gene expression level, and provides the significance through permutation test (1000 times). Only receptors and ligands expressed in more than 25% of the cells in the specific cell subsets were considered in each...