Isolation of high-quality RNA directly from tissues is desirable to obtain precise information of in vivo gene expression profiles in cells embedded within their extracellular matrix (ECM). It is well known that purification of RNA from cartilage tissues is particularly challenging due to low cell ...
While frozen solid, the tissue pieces are ground into a powder with a mortar and pestle. The powder is then processed according to the RNA isolation protocol. However, RNA can still be degraded if the dissection takes too long or the frozen tissue thaws. RNAla...
描述: 使用我们的Multi-Enzymatic Liquefaction of Tissue (MELT™) Total RNA Isolation System时,第一步就是新鲜或冷冻组织的快速酶解——无需额外的研磨步骤。 您的数据:鉴定患者FFPE样品中与癌症相关的mRNA特征描述:H. Lee Moffitt癌症中心和研究所(佛罗里达州坦帕市)的Yeatman实验室研究了结肠癌、乳腺癌和其他...
The isolation of high qualityRNAfrom cells grown in culture is generally far easier to accomplish than is an attemptedRNA isolationfrom tissue. The primary reason is that the three-dimensional architecture of the tissue that must be dismantled and removed compared to disruption of a cell monolayer...
BMC Molecular Biol (2017) 18:22 DOI 10.1186/s12867-017-0099-7 BMC Molecular Biology METHODOLOGY ARTICLE Open Access An optimised protocol for isolation of RNA from small sections of laser‑capture microdissected FFPE tissue amenable for next‑generation sequencing Parisa Amini1†...
The following protocol is designed for small and large tissue samples (tissue volume 10-200 μl), which normally yield about 10-500 μg of total RNA. Materials for total RNA isolation TRIzol Reagent (40% w/w Phenol (saturated at pH 4.3), 1 M guanidine thiocyanate, 1 M ammonium ...
Finally, stay clear of pipettes and laboratory benches used for plasmid minipreps and genomic DNA isolations. These methods typically use high quantities of RNases. When the RNA is of good quality but the yield is low, there are several things to consider. The starting tissue could have ...
isolationThe isolation of high‐quality RNA from the intervertebral disc and especially from the nucleus pulposus is challenging due to the low cellularity and high proteoglycan content of this tissue. In this study, we report a simple modification of the standard guanidinium thiocyanate‐phenol‐...
Rapid, small-scale RNA isolation from tissue culture cells A rapid and simple protocol for the isolation of RNA from transfected tissue culture cells is described. The protocol employs a guanidinium thiocyanate/phe... WQ Xie,LI Rothblum - 《Biotechniques》 被引量: 401发表: 1991年 Development of...
Total RNA Isolation Kit (Tissue) For research use only Sample: up to 30 mg of tissue, up to 25 mg of paraffin-embedded tissue Yield : up to 30μg Introduction The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for isolation of total RNA from tissue sample...