BMC Molecular Biol (2017) 18:22 Page 6 of 11 Fig. 2 Comparison of workflow of the 'old'(classical) and 'new' protocol for RNA isolation from deparaffinised, stained and laser-capture micro- dissected FFPE tissue. a Workflow of the 'old' RNA isolation protocol, starting with ...
While the above suggested protocol has been tested to be effective, if you are instead looking for a convenient solution for RNA isolation, complete with all buffers and reagents required, we recommend looking at the variety of viral & total RNA extraction kits that we have to offer: Want to...
Protocol for Synthesizing Biotinylated aRNA Protocol for Trichloroacetic Acid (TCA) Precipitation Ribominus Transcriptome Isolation Kit (Yeast and Bacteria) RNAqueous™-96 RNA Isolation Kit Automation Protocol RNA Purification Protocols RNA Structure - Fu...
A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites A protocol is described for rapid RNA isolation from various plant species and tissues rich in polyphenolics and polysaccharides. The method is based on th... E Kiefer,W Heller,D ...
The following protocol is designed for small and large tissue samples (tissue volume 10-200 μl), which normally yield about 10-500 μg of total RNA. Materials for total RNA isolation TRIzol Reagent (40% w/w Phenol (saturated at pH 4.3), 1 M guanidine thiocyanate, 1 M ammonium ...
Add 2 ml of cold 10% TCA (trichloroacetic acid) to the 12 X 75 mm tube containing the remaining 100 µl diluted DNA/RNA. Mix thoroughly and place on ice for 10 minutes. This will precipitate nucleic acids longer than ~20 nucleotides. ...
New to the Protocol Library? A great place to start is to determine whether your lab’s workflow can be handled with our with our Featured or Verified protocols found on the left-hand side of the library. Still didn’t find what you’re looking for? Feel free to reach out to your cus...
equipped with a swinging-bucket rotor at room temperature are needed. The kit can also be used for RNA clean-up prior to any downstream application. Purified RNA is suitable for mRNA isolation (Product R6511, mRNA Enrichment Kit), Northern analysis, RNase protection assays, differential display,...
Protocol for Synthesizing Biotinylated aRNA Protocol for Trichloroacetic Acid (TCA) Precipitation Ribominus Transcriptome Isolation Kit (Yeast and Bacteria) RNAqueous™-96 RNA Isolation Kit Automation Protocol RNA Purification Protocols RNA Structure - Fu...
RNA isolation and quantitative reverse-transcription PCR (RT-qPCR) Total RNA was extracted from cultured hiPSCs at 10 days of culture using RNeasy Plus Universal Mini kit (QIAGEN, Germany). The purity and integrity of the extracted RNA were determined using a NanoDrop One spectrophotometer (Thermo...