(1993). Extended application of an efficient method for RNA isolation from different organisms. Biotechnol Tech 7, 639-644.Felipe, M. S. S., Rogelin, R., Azevedo, M. 0., and Astolfi-Filho, S. (1993) Extended application of an efficient method for RNA iso- lation from different ...
modification of unprocessedtransfer, ribosomal and messeger RNAs, and the assembly of proteins from aminoacid monomers may be performed by RNA-derived catalytic residues in theribosome.Previously researchers have developed methods for the isolation of unnaturalRNA ligands and enzymes from random sequence ...
However, the scope of performing microarray using intact RNA derived from LCM isolated cells is very limited due to the instability of mRNA during the LCM process. To overcome this issue, we estimated limitation of the amount of RNA and optimized the RNA extraction procedure for LCM-derived RNA...
In RNA isolation, in order to increase the yield of isolated RNA using a carrier nucleic acid, such as poly-the A-RNA. The carrier nucleic acid is a subsequent process, such as reverse transcription, which can result in high molecular weight products which may interfere with PCR and gel el...
0.preparation of arm and genomic inserts 第33页/共59页 I 2 cDNA libraries I2-1 mRNA isolation, purification I2-2 Check theRNA integrity I2-3 Fractionate and enrich mRNA I2-4 Synthesis of cDNA I2-5 Treatment of cDNA ends I2-6 Ligation to vector Gene libraries and screening 第34...
The PBMNCs and BM CD34 þ cells were used for the preparation of RNA and proteins. The BM CD34 þ cells were also used for further virus infection. Recombination lentivirus construction and transduction. The self-inactivating transfer vector plasmids pMIRNA1, pSIH1-H1-siLuc-copGFPTM and ...
We describe, for the first time, an efficient protocol based on laser capture microdissection (LCM) for the isolation of human epidermal layers for gene expression profiling using quantitative real-time PCR. Two areas enriched either in basal or granular layers were isolated by LCM. Skin biopsies...
摘要: -Isolation of Turkey coronavirus (TCoV). - Development of the full length genome sequences of TCoV. -Development of novel diagnostic assays for TCoV. -Study the molecular pathogenesis of TCoV出版时间: 2013/07/19 ISBN: 978-1-4665-0566-7 ...
Use of flow cytometry and RT-PCR for detecting gene expression by single cells. Biotechniques. 1996;21:286–91. PubMed CAS Google Scholar Hashimshony T, Wagner F, Sher N, Yanai I. CEL-seq: single-cell RNA-seq by multiplexed linear amplification. Cell Rep. 2012;2:666–73. doi:...
Expression of plant genes during the development of pea root nodules. The RNA was translated in vitro and the translation products were separated by two-dimensional gel electrophoresis. The results show differential expression of... Govers,Gloudemans,Moerman,... - 《Embo Journal》 被引量: 155发...