The developed method using nanoparticles took less time to extract DNA when compared to phenol-chloroform and kit method.Key Words: Bacterial genomic DNA, Escherichia coli K12, Extraction of Genomic DNA, Iron oxidenanoparticles SEM.Ketkar Ketaki...
The PureLink HiPure Plasmid Purification Kits allow isolation of high yields of highly pure ssM13 (single-stranded M13) DNA from bacteria. The kits are designed to efficiently isolate plasmid DNA fromE. coliin 1.5-2.5 hours using anion-exchange columns without the use of...
Strains that did not agglutinate with any of the sera were regarded as non-typable (NT). Triplex PCR, HRM and sequencing Genomic DNA was isolated with the Genomic Mini kit (A&A Biotechnology, Gdynia) for isolating genomic DNA from bacteria, cell cultures and solid tissues. DNA was ...
The 16S rRNA and gyrB genes were used to further identify the strain of bacteria isolates. Genomic DNA of each isolate was extracted using the PureLink Genomic DNA Mini Kit (Invitrogen, USA) according to manufacturers’ instructions. The partial 16S rRNA gene was amplified and sequenced using the...
The Escherichia coli rRNA operon rrnB was used as a 32P-labeled hybridization probe in Southern blots of genomic DNAs from representative strains of the saccharolytic, gram-negative, obligate anaerobes of the genus Bacteroides. Control e... CJ Smith,DR Callihan - 《Journal of Clinical Microbiolog...
Escherichia coli (E. coli) is one of the most common uropathogenic bacteria. The emergence of multi-drug resistance among these bacteria resulted in a worldwide public health problem which requires alternative treatment approaches such as phage therapy.
1 998 bp DNA sequence was isolated here with genomic DNA of Antheraea pernyi by Thermal Asymmetric Interlaced PCR(TAIL-PCR).The results from comparing 1 998 bp DNA sequence with the sequence that already landed on the Genebank(AF083334)showed that the DNA sequence(1 758 bp)in the newly iso...
Protocol 13: Isolation of High-Molecular-Weight DNA from Mammalian Cells Using Proteinase K and Phenol Protocol 14: A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues Protocol 15: Preparation of Genomic DNA from Mouse Tails and Other Small Sampl...
In the construction and characterization of yeast artificial chromosome (YAC) contigs, it is necessary to be able to isolate and map the ends of the genomic inserts. This is important with respect to both extending contigs, and identifying chimerism. A number of techniques have now been described...
通过BLASTp蛋白序列分析,噬菌体1A11的70个ORF (表2)中具有与噬菌体结构和结构组装、DNA复制和调控、穿孔素和裂解酶等有关的基因,不含抗生素抗性基因和毒力基因,不会造成跨物种的基因转移,具有用于屎肠球菌噬菌体治疗的潜在价值。 Table 2 ORF analysis of the 1A11 genome ORF Start−End Top BLAST hit Ident...