The developed method using nanoparticles took less time to extract DNA when compared to phenol-chloroform and kit method.Key Words: Bacterial genomic DNA, Escherichia coli K12, Extraction of Genomic DNA, Iron oxidenanoparticles SEM.Ketkar Ketaki...
DNA was isolated from cultured mammalian cells, liver tissue and Escherichia coli after treatment with the common laboratory fixatives for various periods of time. The cultured cells included the mouse cell strain LM and the Chinese hamster cell strain Don C. Liver tissue was obtained from young ...
Incomplete cutting of chromosomal DNA with restriction endonucleases, or poor transformation of Escherichia coli with plasmid DNA from yeast are the difficulties most frequently encountered. The procedures given herein have been chosen since they have used successfully by many workers to obtain DNA that ...
Appl. E... A pseudorandom genomic library of Bacteroides succinogenes DNA, cloned into pUC8 in Escherichia coli, was screened for beta-glucanase activity on 0.1% lich... J E Irvin,R M Teather - 《Applied\\&\\environmental Microbiology》 被引量: 66发表: 1988年 Cloning and Sequencing ...
Strains that did not agglutinate with any of the sera were regarded as non-typable (NT). Triplex PCR, HRM and sequencing Genomic DNA was isolated with the Genomic Mini kit (A&A Biotechnology, Gdynia) for isolating genomic DNA from bacteria, cell cultures and solid tissues. DNA was ...
The PureLink HiPure Plasmid Purification Kits allow isolation of high yields of highly pure ssM13 (single-stranded M13) DNA from bacteria. The kits are designed to efficiently isolate plasmid DNA fromE. coliin 1.5-2.5 hours using anion-exchange columns without the use of ...
Escherichia coli (E. coli) is one of the most common uropathogenic bacteria. The emergence of multi-drug resistance among these bacteria resulted in a worldwide public health problem which requires alternative treatment approaches such as phage therapy.
The 16S rRNA and gyrB genes were used to further identify the strain of bacteria isolates. Genomic DNA of each isolate was extracted using the PureLink Genomic DNA Mini Kit (Invitrogen, USA) according to manufacturers’ instructions. The partial 16S rRNA gene was amplified and sequenced using the...
During the early stages of development, a highly soluble and bioavailable source of calc... MH Henry 被引量: 0发表: 2014年 Nested polymerase chain reaction for detection of Ehrlichia risticii genomic DNA in infected horses. A nested polymerase chain reaction was developed for amplifying a 529-...
Enhanced Automated Immunomagnetic Separation (eAIMS) for Escherichia coli O157 Extraction of DNA using DNAzol Reagent Extraction of DNA From Plants Using Plant DNAzol Reagent How to Use Phenol-Chloroform for DNA Purification Genomic DNA Extraction - PureLink Purificat...