A very simple and inexpensive method for extracting bacterial genomic DNA was developed. A comparison was also made using conventional phenol-chloroform method and commercially available DNA extraction kit to check for the amount of DNA obtained. The developed method using nanoparticles took ...
After preparing the media, for isolation of several bacterial strains containing OPH enzyme that are capable of biodegrading organophosphorus pesticides by diazinon enzymatic hydrolysis, bacterial genomic DNA extraction, plasmid product sequencing, phylogenetic sequence processing and phylogenetic tree drawing ...
Each fresh cultured bacterial colony was spotted onto the target plate (Bruker), air dried, and overlaid with 1 μL matrix solution mixed with an organic solvent solution made of 50% acetonitrile (Sigma Aldrich, Canada) and 2.5% trifluoroacetic acid (VWR, Canada). The target plate was then...
Separation of bacterial DNA from human DNA in clinical samples may have an important impact on downstream applications, involving microbial diagnostic systems. We evaluated two commercially available reagents (MolYsis®, Molzym GmbH & Co. KG, Bremen and Pureprove®, SIRS-Lab GmbH, Jena, both Ge...
NucleoSpin DNA Stool is designed for purification of genomic DNA from bacterial and epithelial cells present in stool samples. The isolated DNA is used for analysis of the microbiome or genotyping of the donor. The kit utilizesMN Bead Tubes Type A(ceramic beads; included in the kit) in combin...
Simultaneous isolation of Ixodidae and bacterial (Borrelia spp.) genomic DNA . Curr Protoc Microbiol Chapter 1 :Unit 1E.2. doi:.10.1002/9780471729259.mc01e02s19 [ Cross Ref ]Jutras, B. L., Liu, Z., and Brissette, C. A. (2010) Simultaneous isolation of Ixodidae and bacterial (Borrelia...
Real-time PCR was performed in a 20 μl reaction mix containing 2 μl of bacterial genomic DNA, SYBRPremix Ex Taq, ROX Reference Dye II (Takara Bio, Otsu, Japan), and each primer. Universal (forward, 5′-AGT CAC TGA CGA GTT TGA TCM TGG CTC AG-3′; reverse, 5′-CAG TGA CT...
此试剂盒设计用于从2亿个活细菌细胞(0.5和1.0 mL培养物)之间快速制备基因组DNA。该试剂盒可用于革兰氏阴性和革兰氏阳性细菌,包括大肠杆菌和蜡状芽孢杆菌。纯化的基因组DNA具有优异的质量和产量,与限制酶消化,测序,PCR,qPCR等应用完全兼容。还提供96孔形式,适用于高通量应用。
mutations in the ribosomal protein L3 region and the acquisition of the adenosine triphosphate-binding cassette transporter transposon-borne gene (vgaAv variant... LPH Yang,SJ Keam - 《Drugs》 被引量: 26发表: 2008年 Melazocine masticatory and its preparation Fungal, bacterial, and cancer cells ...
Thermo Fisher Scientific offers Genomic DNA extraction kits in your choice of formats for specific sample types: Tissue DNA extraction kits Blood DNA extraction kits Cell DNA extraction kits Plant DNA extraction kits Forensic DNA extraction kits Biopharmaceutical DNA extraction kits Bacterial DNA ...