Shiroza T, Shinozaki N, Watanabe T, Ikemi T, Fukushima K, Abiko Y. Rapid isolation of chromosomal DNA from oral streptococci and polymerase chain reaction-oriented restriction fragment-length polymorphism analysis for genetic heterogeneity. Oral Microbiol Immunol. 1998;13:11-6....
A rapid alkaline extraction method for the isolation of plasmid DNA Plasmids are double-stranded circular DNA molecules that have the property of self-replication, independent of chromosomal DNA. In bacteria, they carry gen... BIRNBOIM,H C - 《Methods in Enzymology》 被引量: 2224发表: 1983年...
A simple procedure for large-scale preparation of pure plasmid DNA free from chromosomal DNA from bacteria A very simple, inexpensive procedure for preparing pure plasmid DNA from bacteria is described. In this method, lysozyme-induced spheroplasts are made in p... M Mukhopadhyay,NC Mandal - 《...
Antibiotic resistance genes are often carried by plasmids, which spread intra- and inter genera bacterial populations, and also play a critical role in bacteria conferring phage resistance. However, it remains unknown about the influence of plasmids pres
Wanted Bacteria by Metaparental Mating Páraic Ó Cuív1,2, Wendy J. Smith1, Sian Pottenger2, Sriti Burman2, Erin R. Shanahan2,3 & Mark Morrison1,2 Metagenomics has rapidly advanced our inventory and appreciation of the genetic potential inherent to the gut microbiome...
Method for isolation of extrachromosomal amplified 优质文献 相似文献 参考文献 引证文献General Method for the Isolation of Plasmid Deoxyribonucleic Acid Plasmid deoxyribonucleic acid (DNA) ranging from 5 x 10(6) to 65 x 10(6) daltons may be isolated from chromosomal DNA by the preferential precipitat...
6.Clear the lysate of precipitated SDS,proteins,membranes,and chromosomal DNA by pouring through a double-layer of cheesecloth.Transfer the lysate into 250 ml centrifuge bottle,centrifuge at 10,000 rpm for 30 minutes at 4deg C in the RC5-B using the GSA rotor. ...
A novel method for rapid isolation of plasmid DNA A new disposable chromatographic column, pZ523, has been developed for separating plasmid DNA from bacterial chromosomal DNA. Use of pZ523 spun columns eli... PH Zervos,LM Morris,RJ Hellwig - 《Biotechniques》 被引量: 24发表: 1988年 ...
A key feature that differentiates prokaryotic cells from eukaryotes is the absence of an intracellular membrane surrounding the chromosomal DNA. Here, we isolate a member of the ubiquitous, yet-to-be-cultivated phylum ‘Candidatus Atribacteria’ (also kn
Briefly, 1 mL of phages was mixed with 2.5 × 108 colony forming units (CFU) mL−1 of bacteria in 9 mL at a multiplicity of infection (MOI) of 0.001. The mixture was incubated at 37 °C with shaking and samples withdrawn each 1 min for titration. In addition, we performed a one...