CTAB DNA Extraction Protocol This protocol was used in the Kalisz Lab for the Outcrossing Rates/Microsat project from 2006 on. Adapted for the Kalisz lab from Soltis lab protocol and Doyle & Doyle, 1987. Before Beginning: Turn on the waterbath. Double check that it is set to 55C and the...
Cadaveric DNA extraction protocol based on cetyl trimethyl ammonium bromide (CTAB). In: Mangin P, Ludes B, editors. Acta Medicinae Legalis Vol XLIV 1994. Springer Verlag 1995; 35-6.Corach D, Penacino G, Sala A (1994) Cadaveric DNA extrac- tion protocol based on cetyl trimethyl amonium ...
The protocol is also efficient for qu ick and macro DN A extraction.Key words:Actinidia ; genomic DNA; isolation; Southern blot DNA 保存方式包括 DNA 分子保存( 即 DNA 库保存) 、 基因组文库保存、 cDNA 文库保存和特殊基因片段保存等。采用 DNA 保存, 不仅具备其他资源保存方法的一般特点, 还具有...
关键词 改进的CT AB法;盐生植物;DNA提取 中图分类号:Q946.2 文献标识码:A M od i f i ed CTAB Protocol for Extracti n g the Tot a l D NA of Ha lophyte W ang Yanqin Luo Xiaoxia L iu Chen L i Xia Chen L iang Yang J ian (Key Laborat ory of Pr otecti on and U ...
Unlike the animal cell, DNA extraction from plant cells faces challenges of a rigid cellulose cell wall and the presence of a variety of cytoplasmic components like polysaccharides, polyphenols, lipids, and proteins. This demands modification in the DNA extraction protocol to obtain DNA in the pures...
CTAB DNA Extraction protocolCTAB DNA Extraction Protocol This protocol was used in the Kalisz Lab for the Outcrossing Rates/Microsat project from 2006 on. Adapted for the Kalisz lab from Soltis lab protocol and Doyle & Doyle, 1987. Before Beginning: Turn on the waterbath. Double check that ...
Examines a study conducted on the use of a consistent protocol for extracting DNA from expanded leaf materials containing large quantities of polyphenols, tannis and polysaccharides. Methodology based on the involvement of a modified cetyltrimethlammoniumbromide (CTAB) extraction which employed high salt...
The protocol has been used successfully for DNA purification from rumen fluid and plant cells. Moreover, after slight alterations, the same protocol was used for large-scale extraction of DNA from pure cultures of Gram-positive and Gram-negative bacteria. The yield of the DNA obtained with this...
There have yet to be reports regarding effective and efficient soybean genomic DNA extraction methods. This study aimed to obtain a rapid extraction method for soybean with high-quality DNA. This study used a modification of the CTAB method by shortening the duration its stages. The treatments ...
Considering the need to develop genomic studies with these genotypes and minimize costs in the genotyping process, in this research, five CTAB DNA extraction protocols (P1-P5) were evaluated and compared with a commercial kit. Four local rice accessions from the CPGB were used, and DNA ...