2. Li, Y. X., Zhang, Z. J., Ye, J. H., Deng, X. L., Yu, X. D., Li, X. M., … & Fang, Z. Y. (2014). A simple and efficient CTAB‐method for genomic DNA extraction from Lupinus spp. (Fabaceae) leaves. Genetics and Molecular Research, 13(4), 8542-8551....
1.Grind the tissue or cells that you want to extract DNA from using a mortar and pestle or other mechanical method. 2.Add a lysis buffer (usually containing CTAB and other reagents) to the ground tissue or cells and incubate at a high temperature (usually around 65-70°C) to denature ...
A new method CTAB-silica for DNA extraction and purification from the leaves and buds of Castanea mollissima and Ginkgo biloba was tested. The method is based on the silica-based purification protocol developed by Boom et al. (1990). By modifying the protocol, plant genome DNA could be ...
英文名称Plant Genomic DNA Extraction Kit (CTAB rough extraction method) 英文同义词 CAS号 分子式 分子量0 EINECS号 相关类别 Mol文件Mol File 结构式 植物基因组DNA提取试剂盒(CTAB粗提法) 性质 植物基因组DNA提取试剂盒(CTAB粗提法) 用途与合成方法 ...
makingitasystematicmethodinthelabteaching.Andthus,CTAB methodwasamethodnotonlyusedinresearch,butalsousedinlargescalelabteaching. Keywords:DNAextraction;CTABmethod;AnimalgenomicDNA 1引言 长久以来,动物基因组DNA的提取一直采用SDS-蛋白酶 心管中,加入等体积的氯仿-异戊醇(24:1),轻轻振荡混 匀,于6000rpm的离心机...
Castanea mollossima BL.; DNA extraction; CTAB method; SDS method; AFLP 板栗(Castaneamollissima BL.)是壳斗科栗落叶乔木,是一种重要的园艺作物,其经济栽培价值高[1]。迄今为止,人们对板栗的研究很多,但多集中于板栗的种植、病虫害防治及板栗壳的利用等方面,而对板栗基因组DNA的研究相对较少。基因组DNA的...
Abstract：Objective：To verify with the feasibility of CTAB－improved Methods of DNA Extraction in Genome of the Mouse Muscle. Methods：With the CTAB－improved method，genomic DNA of the fresh mouse thigh muscles was extracted,the purity and content of DNA was analysed by agarose gel ...
1. Grind the tissue or cells that you want to extract DNA from using a mortar and pestle or other mechanical method.©2022 Baidu |由 百度智能云 提供计算服务 | 使用百度前必读 | 文库协议 | 网站地图 | 百度营销
DNA Genomic DNA Extraction from Phalaenopsis hybrid by CTAB Method 利用CTAB法提取蝴蝶兰的基因组 www.ilib.cn 2. DNA Isolation of genomic DNA from different organs in Anthurium andraeanum by CTAB method 利用CTAB法提取红掌不同部位基因组 www.ilib.cn 3. DNA extraction of Staphylococcus aureus using th...
Due to some defects existed in DNA extraction method from animal tissues used in routine lab work,an improved CTABwas used in genomic DNA extraction from animal tissues.The results indicated that the improved CTAB method had privileges ofshort time span,higher quality of DNA product,low cost,and...