The method involves extraction of DNA using a buffer (pH 8.0) containing Tris HCL (100 mM), EDTA (20mM), 5 M NaCl, 10% CTAB and 2% 0- mercaptoethanol, followed by purification of DNA with phenol, chloroform and Isoamly alcohol and finally precipitation of DNA by sodium acetate and ...
Nucleic acid extraction and PCR amplification Total DNA was extracted in triplicate from each cecal sample using a modified cetyltrimethylammonium bromide (CTAB) extraction method as previously described6. This method uses the ionic detergent CTAB to disrupt cell membranes and a chloroform-isoamyl alcoho...
Genotyping of individuals plays a pivotal role in various biological analyses, with technology choice influenced by multiple factors including genomic constraints, number of targeted loci and individuals, cost considerations, and the ease of sample prepa
Microwave synthesis and effect of CTAB on ferromagnetic properties of NiO, Co3O4 and NiCo2O4 nanostructures Cubic-structured NiO, Co 3 O 4 and spinel-structured NiCo 2 O 4 were synthesized via microwave route. The structural properties of NiO, Co 3 O 4 and NiCo 2 O 4 nanostructures were...
Ethanol pretreatment increases DNA yields from dried tree foliage of plant H, Ehwald R (2010) Extraction of nucleic acids from yeast cellsand plant tissues using ethanol as medium for sample preservation and cell CTAB... Akinnagbe,XB,Yang,... - 《Conservation Genetics Resources》 被引量: 4发...
2), F1 blades developed from conchospores, and F1 blades developed from archeospores, all of which were cultured as part of the previous crossing experiment with HG-4 and IBYC-G1 (Niwa et al., 2018), were used for DNA extraction. Total DNA was extracted by the modified CTAB method II...
DNA extraction used a three-step procedure including lysis in CTAB buffer, phenol-chloroform purification and isopropanol precipitation, following Perrot-Minnot (2004). PCR reactions were performed in a final volume of 10 μL containing 1 μL template DNA, 200 mM of each nucleotide, 5...
To further verify the purity of the isolated strains, genomic DNA of three independently grown cultures of each strain was extracted with a genomic DNA extraction kit (Exgene Soil DNA mini, GeneAll, Korea) according to the manufacturer’s instructions. DNA of each sample was used as templates ...
Then, the reaction sample was purified by phenol extraction or CTAB precipitation. However, the protocol described above gave only insufficient results. In contrast to the above protocol we now added different concentrations of magnesium chloride. At the same time the 10×Sequitherm® reaction ...
This invention is intended to develop many DNA markers in plants of the genus Fragaria and to evaluate anthracnose resistance with high accuracy with the use of such many DNA