The method comprises the following steps: (1) extracting DNA of a sample to be detected, namely, adding a CTAB extraction buffer solution into the sample to be detected, and extracting DNA by using a common CTAB method; (2) establishing an LAMP system, namely, preparing a 25[mu]l ...
DNA extraction from all fractions was carried out using the CTAB method50. Total 16S rDNA was amplified from each fraction using 8F and 530R universal primers for bacteria51 using multiplex identifiers (MIDs, SCIENTIFIC REPORTS | 3 : 3515 | DOI: 10.1038/srep03515 7 www.nature.com/...
Fecal DNA was extracted with the standard protocol for genomic DNA extraction with CTAB (Cetyl Trimethyl Ammonium Bromide). DNA concentration was measured using Qubit dsDNA Assay Kit in Qubit 2.0 Fluorometer (Life Technologies, CA, USA). For shotgun sequencing, Illumina libraries were prepared using...
Triplicate qPCR reactions were performed for each sample, and the relative gene expression data were analyzed using the 2−ΔΔCt method59. Southern blot and Western blot analysis DNA was extracted from leaves of the putative transgenic eggplant lines using the CTAB method, and Southern blot ...
2.3. DNA extraction and genotyping Fresh young leaves were collected from each accession for DNA extraction using a modified CTAB method [22]. The quality and concentration of the extracted DNA were assessed via agarose gel electrophoresis and a NanoDrop UV–Vis spectrophotometer, respectively. The ...
DNA Extraction DNA was extracted between three and seven times from 0.3 g Bio-Sep beads (depending on yields) following a modified CTAB bead-beating procedure in tubes with three 5 mm glass beads (DeAngelis et al., 2010). To clear residual phenol, all extractions for...
2.5 Molecular characterization: Bacterial DNA was extracted from bacterial cells using CTAB (Cetyl-trimethyl- ammonium-bromide) method [18]. The bacterial cells were resuspended in warm extraction buffer (20 mM EDTA, pH 8.0, 1.4 M NaCl, 10 mM Tris–HCl pH 8.0, 3% CTAB and 0.3% ...
CTAB/NaCl incubation was followed by phenol/chloroform/isoamyl alcohol (25:24:1), then a chloroform/isoamyl alcohol extraction step. DNA was quantified using Quant-iT PicoGreen assay (Invitrogen, Life Technologies) and 1 ng of gDNA was used as a template to construct the sequencing library,...
Pre-FMT home stool sample collection was facilitated by patients and shipped overnight to the investigators. Upon receipt, samples were frozen at -80°C. Fecal DNA extraction was performed using a modified cetyltrimethylammonium bromide (CTAB) method as previously described (Selvig et al., 2020)...
DNA extraction and PCR amplification We used the CTAB/SDS method to extract total genomic DNA from all the soil samples. DNA concentration and purity were measured using a 1% agarose gel. Depending on the concentration, DNA was diluted to 1 ng μL−1using sterile water. The V4 regions of...