Genomic DNA obtained with the CTAB-PEG method had high integrity, with average fragment size >30 kb, the concentration higher than 100 ng/渭L, and the yield more than 30 渭g/g. Presented technique is suitable for DNA extraction from fungi, bacteria, archaea or even mollusks with high ...
冷冻研磨CTAB法2) CTAB method 研磨-CTAB法 1. Compare Analysis of CTAB method with alkaline guanidine thiocyanate boiling method for extraction of fungal genomic DNA; 研磨-CTAB法与碱性异硫氰酸胍沸腾法提取真菌DNA的比较分析3) Refrigeration-rubbing SDS 冷冻研磨SDS法...
提 取的基因组DNA可用于基因组文库构建、PCR等分子生物学实验。 关键词 CTAB DNA提取 多糖去除 中图分类号 Q89 齐整小核菌(Sclerotiumrolfsii)是一种土壤植物病 原真菌,能导致农业和园林作物患白绢病⋯。但该菌 在工业上用来大量生产硬葡聚糖。硬葡聚糖是一种中 性的水溶性多聚物,主链由8—1,3-D一吡喃葡萄...
Study on Efficient Extraction for Genomic DNA from Malus Leaves by CTAB Method CTAB法高效提取苹果叶片DNA的研究 2. Method The samples were extracted by the classic technique of CTAB and improved CTAB. 方法采用经典CTAB法与改良CTAB法。 3. We adopted the phenol-chloroform-isopentanol, CTAB and ...
Fungal DNAOUT 常温 一年 酵母DNAout 690 50次 柱式真菌DNAout 990 50次 Column Fungal DNAOUT 常温 一年 公司简介 上海钰博生物科技有限公司是2012年5月由“海归”组成的创业团队与两个专业投资机构发起成立的,以开发生产科研试剂和特色检验产品为方向,经过两年多的努力,已经建立了免疫学和分子生物学的五个技术平...
(ii) For DNA extraction from basidiomata of herbarium specimens and frozen field-collected specimens with a high polysaccharide content, CTAB is definitely the method of choice. 29 A. Góes-Neto et al. Acknowledgements We would like to thank all those who contributed directly or indirectly to ...
Fresh samples of unknown variety were collected in 2019 from a farmer’s field in Kimara in Dar Es Salaam and stored at 4 °C for laboratory analysis on the same day. Isolation of genomic DNA and total RNA Three CTAB-based extraction methods referred to in this paper as Method 1 (M1),...
SDS_CTAB法提取烟草病圃土壤微生物总DNA
3、mplifi-ThesizeoftheobtainedDNAafterpurificationwasabout21kb,cationwithbacterialandfungalconservedsequenceprimersshowedthattheamplifiedbandswiththeexpectedsizecouldbedetectedbyagarosegelelectrophoresisKeywords:Tobacco;Diseasedfield;Microbesinsoil;TotalDNA;SDSCTABmethod土壤微生物包含细菌、真菌和病毒等生物,具有多样性和...
The present study reports a fast and reliable method for quality DNA extraction from the fungus Blumeria graminis f. sp. tritici (Bgt), the cause of powdery mildew of wheat, for use in molecular studies. DNA was isolated directly from fungal spores using cetyl trimethylammonium bromide (CTAB)...