Genomic DNA obtained with the CTAB-PEG method had high integrity, with average fragment size >30 kb, the concentration higher than 100 ng/渭L, and the yield more than 30 渭g/g. Presented technique is suitable for DNA extraction from fungi, bacteria, archaea or even mollusks with high ...
11. 每个样品用2050 L TE溶解DNA,并添加12 L无DNase的RNase A(10 mg/mL)。37°C孵育30分钟。 12. 确定浓度后,贮存于4°C(或长期贮存于20°C)。Small-scale Preparation of Plant Genomic DNA Using CTABPrinciple The CTAB method of DNA isolation was initially used in bacteria and 6、later ...
TiedjeJMDNArecoveryfromsoilsofdi-AppliedandEnvironmentalMicrobiolo-versecompositionJgy,1996,62:31632213胡磊,杨广,尤民生茶园土壤微生物总DNA不同提取方法J基因组学与应用生物学,2010(2):361368的比较14CullenDW,HirschPRSimpleandrapidmethodfordirectJSoilBiolextractionofmicrobialDNAfromsoilforPCRBiochem,1998,30:983993...
SDS_CTAB法提取烟草病圃土壤微生物总DNA
Genomic DNA obtained with the CTAB-PEG method had high integrity, with average fragment size >30 kb, the concentration higher than 100 ng/渭L, and the yield more than 30 渭g/g. Presented technique is suitable for DNA extraction from fungi, bacteria, archaea or even mollusks with high ...
This method is basically introduced for Universities where there is time limit, Research centre's where DNA extraction has to be quick enough with good quality and intensity in just one go and also can be used in High schools where students can easily handle with no risk of injury and ...
DNARNAvirusmarine ecosystemKarl and Bailiff (1989) presented the CTAB-DABA-orcinol method for the determination of dissolved DNA (D-DNA) and RNA (D-RNA) in natural waters. Here we demonstrate that, by this method, no only RNA but also heteropolysaccharides containing pentose sugars are ...