1、CTAB法小量制备植物基因组DNA【原理】 CTAB分离DNA的方法最初用于细菌,后来经修改用于从植物中获取DNA。CTAB(即十六烷基三乙基溴化铵)是一种阳离子去污剂。CTAB可与核酸形成复合物,该复合物仅溶于高盐溶液。此外,CTAB可十分有效地促进DNA和RNA与多糖的分离并去除多糖。可通过提高NaCl的浓度和沉淀核酸去除CTAB。
CTAB法小量制备植物基因组DNA CTAB法小量制备植物基因组DNA 【原理】 CTAB分离DNA的方法最初用于细菌，后来经修改用于从植物中获取DNA。CTAB（即十六烷基三乙基溴化铵）是一种阳离子去污剂。CTAB可与核酸形成复合物，该复合物仅溶于高盐溶液。此外，CTAB可十分有效地促进DNA和RNA与多糖的分离并去除多糖。可通过提高...
The principle modifications currently employed for DNA extraction involved the use of higher CTAB concentration and higher levels of 2-尾-mercaptoethanol. Additionally, higher concentrations of sodium chloride and potassium acetate were added simultaneously with absolute ice cold isopropanol for the ...
⒈DNA fragmentation for second-generation sequencing samples ⒉RNA fragmentation ⒊Bacterial and cell lysis ⒋ChIP assay (Chromatin Immunoprecipitation) ⒌Preparation of samples for high-throughput sequencers ⒍Extraction of membrane proteins/proteins ⒎Homogenization, emulsification reactions ⒏Ultrasound treatment...