DNA gyrase is an essential enzyme involved in the homeostatic control of DNA supercoiling and the target of successful antibacterial compounds. Despite extensive studies, a detailed architecture of the full-length DNA gyrase from the model organism E. co
The severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne human-infecting bunyavirus, which utilizes two envelope glycoproteins, Gn and Gc, to enter host cells. However, the structure and organization of these glycoproteins on virion surface are not yet known. Here we describe...
g, Representative cryo-EM density for different parts of the C-terminal domain of PvRBP2b. Selected residues are also indicated. h, FSC curves of the final refined model versus the final cryo-EM map (full dataset, blue), of the outcome of model refinement with a half map versus the ...
Higher resolution, however, has not been the only improvement. The proteins studied by single-particle cryo-EM have also become increasingly smaller. The lower size MW limit broke the 100-kDa barrier in 2016 with a structure of the 93-kDa-sized cancer-associated isocitrate dehydrogenase in comple...
Figure 1. The αvβ8 Integrin Ectodomain Bound to L-TGF-β1 (A) Cryo-EM density map of αvβ8 integrin ectodomain with L-TGF-β1 bound. The map is displayed as unsharpened and at a low threshold. The color code is as follows: integrin αv subunit is green, integrin β8 subunit...
The well-resolved tertiary structures in our cryo-EM maps allowed us to survey 21,615 AlphaFold2-predicted protein models of the mouse proteome and unbiasedly identify matching ones. Such a visual proteomics approach helped us to discover novel microtubule-associated proteins in mammalian sperm, ...
Rix7 is an essential type II AAA-ATPase required for the formation of the large ribosomal subunit. Rix7 has been proposed to utilize the power of ATP hydrolysis to drive the removal of assembly factors from pre-60S particles, but the mechanism of release
The Tweety homologs (TTYHs) are members of a conserved family of eukaryotic membrane proteins that are abundant in the brain. The three human paralogs were assigned to function as anion channels that are either activated by Ca2+ or cell swelling. To unco
The 26S proteasome is an ATP-dependent dynamic 2.5 MDa protease that regulates numerous essential cellular functions through degradation of ubiquitinated substrates. Here we present a near-atomic-resolution cryo-EM map of the S. cerevisiae 26S proteasome
1 Time-resolved cryo-EM of translocation with EF-G and GTP. a Scheme of the translocation reaction of the 70S•mRNA•fMet-tRNAfMet•Pro- tRNAPro complex with EF-G•GTP. b Segmented cryo-EM maps of 8 states of the translocation reaction, and their assignment as substrates, EF-G-...