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Single particle cryo em is a powerful structural biology technique for 3D characterization of proteins, protein complexes and macromolecules at near-atomic and atomic resolution.
Single particle cryo em is a powerful structural biology technique for 3D characterization of proteins, protein complexes and macromolecules at near-atomic and atomic resolution.
CryoEM Processing|CryoEM 数据处理-transfer cryoSPARC single particle to RELION 单颗粒冷冻电镜数据处理中用的比较多的软件是RELION和CryoSPARC。两者各有利弊,且不同的人有不同的使用倾向。笔者认为,RELION的MotionCorr和Polish较好,而CryoSPARC的2D,Nu-refinement则较优。对于有些比较难处理的数据集,有些人会将两...
Electron cryo-microscopy (cryo-EM) single-particle analysis has yielded protein structures with increasing levels of detail in recent years1,2. However, it has proved difficult to obtain cryo-EM reconstructions with sufficient resolution to visualize individual atoms in proteins. Here we use a new ...
程亦凡《Structural Biology in the Era of Single-Particle CryoEM》是直播回放 | 结构+AI: 冷冻电镜与药物创新发现论坛的第2集视频,该合集共计14集,视频收藏或关注UP主,及时了解更多相关视频内容。
micrographs of rotavirus and HIV-1 virus-like particles were imaged using ptychography reporting the principal applicability to biological specimens albeit at low resolution27. Thus far, complete three-dimensional (3D) single-particle cryo-EM structures of biological macromolecules at close-to-atomic reso...
Cryo-electron microscopy (cryo-EM) of biological molecules in single-particle (i.e., unordered, nonaggregated) form is a new approach to the study of molec... Frank,Joachim - 《Annual Review of Biophysics & Biomolecular Structure》 被引量: 497发表: 2002年 Single-Particle Cryo-Electron Micros...
Single-Particle Cryo-Electron Microscopy (Cryo-EM)http://linkinghub.elsevier.com/retrieve/pii/B9780128001448000021doi:10.1016/B978-0-12-800144-8.00002-1David AgardYifan ChengRobert M. GlaeserSriram SubramaniamAdvances in Imaging and Electron Physics...
内容提示: REVIEWSingle-particle cryo-EM—How did itget here and where will it goYifan ChengCryo–electron microscopy, or simply cryo-EM, refers mainly to three very different yetclosely related techniques: electron crystallography, single-particle cryo-EM, andelectron cryotomography. In the past ...