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Single particle cryo-EM sample preparation workflow A sample for single particle analysis is typically a vitrified suspension of biological material consisting of proteins, protein complexes, viruses or other macromolecules. As sample preparation is a vital step in the sing...
For researchers using the cryo-electron microscopy (cryo-EM) single-particle analysis workflow, our Thermo Scientific Accelerate and Advance Service portfolios offer intensive on-site and remote service and training throughout the lifetime of your workflow. Service at Thermo Fisher Scientific is a part...
Cryo-electron microscopy combined with single-particle reconstruction is a promising technique for solving the high-resolution structure of macromolecular complexes, even in the presence of conformational or compositional heterogeneity. However, the usual workflow leading to one or several structures is ...
Here we present a workflow that combines the advantages of single-particle cryo-EM and StA with a focus on achieving subnanometer resolution. Our workflow consists of recording a higher dose micrograph at zero-degree tilt followed by recording the remainder of the tilt series in the standard way...
we here report the successful subnanometer single-particle cryo-EM structure at 6.5 Å resolution. More systematically, using iDPC–STEM imaging of TMV, we further demonstrate the successful single-particle based helical reconstruction at 3.5 Å resolution using an electron beam of 4.0 mrad...
Single-Particle Cryo-EM at Crystallographic Resolution Until only a few years ago, single-particle electron cryo-microscopy (cryo-EM) was usually not the first choice for many structural biologists due to its l... Yifan,Cheng - 《Cell》 被引量: 167发表: 2015年 A pipeline approach to single...
CryoPROS is a computational framework specifically designed to tackle misalignment errors caused by preferred orientation issues in single-particle cryo-EM. It addresses these challenges by co-refining synthesized and experimental data. By utilizing a self-supervised deep generative model, CryoPROS synthesi...
A recent single-particle cryo-EM study that required forced MET dimerization by the addition of a leucine-zipper motif reported two distinct structures for MET dimers bound to the ligands HGF and NK117. Interestingly, one HGF ligand was found to be sufficient to dimerize two MET receptors, by...
Cryo-EM workflow solutions webinars Play Mute Current Time 0:00 / Duration 0:00 Loaded: 0% SharePicture-in-PictureFullscreen This is a modal window. The Playback API request failed for an unknown reason Error Code: VIDEO_CLOUD_ERR_UNKNOWN Session ID: 2024-12...