S2). A subset of protein particles was manually picked and processed with reference-free 2D classification. Three representative 2D class averaged images were selected as references for automatic particle picking of the entire dataset, which resulted in a total of 343,042 particles. Then the ...
To answer the question of why the mC-hnRNPA2-LCD hydrogel exhibits reversibility, we determined the cryoEM structure of mC-hnRNPA2-LCD fibrils to a resolution of 3.1 Å (Fig.2c). During cryoEM data collection and processing, we found that the mC-hnRNPA2-LCD fibrils have only a singl...
Cryo-EM structure reconstruction (A) Class-averages from the reference-free two-dimensional classification.Hideki ShigematsuKazuko IidaMasataka NakanoPratima ChaudhuriHidetoshi IidaKuniaki Nagayama
Figure 3. Cryo-EM map of homotrimers of MCP p72 purified from the virion at 4.6 Å resolution. A, left, top view of a 2D class-average obtained during 2D classification of ASFV capsomers (composed of MCP p72) unequivocally showing the presence of a pseudo-hexameric symmetry with a true...
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One of the main challenges in cryo-EM is the typically low signal to noise ratio (SNR) of the acquired images. 2D classification of images, followed by class averaging, improves the SNR of the resulting averages, and is used for selecting particles from micrographs and for inspecting the ...
Ultra-fast end-to-end processing of raw cryo-EM data and reconstruction of electron density maps, ready for ingestion into model building software Optimized algorithms and GPU acceleration at all stages, from pre-processing through particle picking, 2D particle classification, 3D ab-initio structure ...
Ultra-fast end-to-end processing of raw cryo-EM data and reconstruction of electron density maps, ready for ingestion into model building software Optimized algorithms and GPU acceleration at all stages, from pre-processing through particle picking, 2D particle classification, 3D ab-initio structure ...
Single particle cryo-electron microscopy (cryo-EM) uses the particles extracted from thousands of images to reconstruct 3D structures of macromolecules. The yield of an acquisition session at the microscope depends on the time needed to set up the run and the speed of the acquisition itself...
Single particle cryo-electron microscopy (cryo-EM) uses the particles extracted from thousands of images to reconstruct 3D structures of macromolecules. The yield of an acquisition session at the microscope depends on the time needed to set up the run and the speed of the acquisition itself...