The percentages of PD-L1+ cells in respective monocyte subsets were compared with respect to different clinicopathological conditions and the association with survival time was assessed.Results: The number of PD-1 positive cells in CD14+ monocytes was very low (almost <1%). Also, the monocyte ...
The third monocyte subset, CD14++ CD16+, is thought to be an intermediate phenotype between CD14++ CD16− classical monocytes and CD14+ CD16+ pro-inflammatory monocytes (34,35). Although they can be classified as three different cells, it is believed that CD14++ CD16− classical ...
which comprise two main subsets: classical (Ly6Chighin mice, CD14+CD16−in humans) and non-classical or patrolling (Ly6Clowin mice, CD14−CD16+in humans) [1]. Classical monocytes can act as precursors to non-classical monocytes, migrate into tissues and further differentiate...
Also, the role of IL-1 and IL10 secreting classical monocytes in early clearance of infection could be explored with in vitro mechanistic studies. 展开 关键词: Tuberculosis TST IGRA TB Resisters Classical Monocytes IL-1ß DOI: 10.1016/j.humimm.2025.111240 年份: 2025 ...
Monocytes are a critical component of the cellular innate immune system, and can be subdivided into classical, intermediate and non-classical subsets on the basis of surface CD14 and CD16 expression. Classical monocytes play the canonical role of phagocy
Fig. 1. Metabolic responses to acute (2 h, 10 ng/mL) LPS-stimulation in glucose-deprived CD14+CD16- classical monocytes. (A). Oxygen consumption response (OCR) to LPS in older and younger subjects. (B). Glycolytic response to LPS. (C). Calculated values for respiratory parameters....
PBMC were washed in PBS (Sigma-Aldrich, Saint Louis, USA) and then monocyte subsets (classi- cal—CD14++CD16−, intermediate—CD14++CD16+ and non-classical—CD14+CD16++) were isolated using flow cytometry cell sorting. The following monoclonal antibodies (mAbs) were used to stain monocytes...
Here, we determined whether the cell surface levels of two markers, pro-inflammatory-related S100A9 and anti-inflammatory-related CD163, expressed on CD14strongCD16− classical monocytes by flow cytometry could discriminate IPF from idiopathic nonspecific interstitial pneumonia (iNSIP). Twenty- ...
Fig. 1 Immune cells-mediating HIV neuroinvasion. To date, four immune cell types are described to support HIV invasion into the CNS. Those include CD14 + CD16 + monocytes, perivascular macrophages, CD4 + T cells, and CD4dimCD8brightT cells. CD4dimCD8brightT cell may invade ...
Classical (CD14++CD16), intermediate (CD14++CD16+), and non-classical (CD14+CD16++) CD86-positive monocytes and...doi:10.1016/j.cardfail.2016.03.009McTiernanCharles F.Elsevier Inc.Journal of Cardiac Failure