Evidence on the changes in the absolute counts of monocyte subpopulations in sepsis is missing. 背景 目前关于脓毒症患者外周血中单核细胞亚群绝对计数的证据是缺失的。 Methods Firstly, absolute counts of circulating CD14pos...
Circulating CD14+CD16+ monocytes, a potent phagocytosing and antigen-presenting monocyte population, have been reported to be expanded in patients on hemod... N Kawanaka,Y Nagake,M Yamamura,... - 《Nephron》 被引量: 59发表: 2002年 Expression of CD14, CD16 and CD45RA on monocytes from...
Evidence on the changes in the absolute counts of monocyte subpopulations in sepsis is missing. Firstly, absolute counts of circulating CD14pos/HLA-DRpos/CD45posmonocytes were measured by flow cytometry in 70 patients with Gram-negative sepsis and in 10 healthy volunteers. In the second phase, ...
MCP-3 ant-120 Mouse Anti Human Macrophage/Monocyte Chemotactic Protein-3/MARC MIP-1 a ant-118 Mouse Anti-Human Macrophage Inflammatory Protein-1a (CCL3) MIP-3 ant-121 Mouse Anti-Human Macrophage Inflammatory Protein-3 MIP-3b ant-213 Mouse Anti-Human Macrophage Inflammatory Protein-3 beta (CCL...
the mice were either challenged with phosphate-buffered saline as mock control or with 5 × 106monocyte-derived dendritic cells (derived from the same donor) pulsed with human CMV viral lysate. Blood samples were drawn on day 7 to determine the cell counts of human CD45+cells. On day 14,...
human brain microvascular endothelial cells (BMVECs) to CD40L increased the expression of adhesion molecules intracellular adhesion molecule-1 and vascular cell adhesion molecule-1, which yielded a fourfold increase in monocyte adhesion to BMVECs and stimulated migration across anin vitroBBB model [128...
In some experiments, CD14+ monocytes were purified from PBMCs by directly conjugated anti-CD14 magnetic microbeads and magnetic cell separation (MACS) columns (both from Miltenyi Biotec, Surrey, United Kingdom) according to the manufacturer's instructions. Purity of the monocyte preparation was ...
We have avoided any manipulations such as carbonyl iron depletion or plastic adherence normally used for monocyte depletion because the abnormal B cells in MM are adherent and are depleted by these procedures. Normal B cells are not depleted and the procedure does not affect the intensity of ...
Briefly, cell aliquots were seeded in Iscove's modified Dulbeccco's medium containing 24% FCS, 1.3% methylcellulose, c-kit ligand (KL; 100 ng/ml), IL-3 (10 ng/ml), G-CSF (10 ng/ml), granulocyte-monocyte (GM)- CSF (10 ng/ml) (all gifts of the Kirin Brewing Co., Gunma, ...