Evidence on the changes in the absolute counts of monocyte subpopulations in sepsis is missing. 背景 目前关于脓毒症患者外周血中单核细胞亚群绝对计数的证据是缺失的。 Methods Firstly, absolute counts of circulating CD14pos...
Evidence on the changes in the absolute counts of monocyte subpopulations in sepsis is missing. Firstly, absolute counts of circulating CD14pos/HLA-DRpos/CD45posmonocytes were measured by flow cytometry in 70 patients with Gram-negative sepsis and in 10 healthy volunteers. In the second phase, ...
In regard to HIV-1 neuropathogenesis, a link between CD40 and microglia has been established. Upregulation of CD40 expression has been detected on microglia of HIV-1-infected brain tissues [28]. CD40L was also shown to potentiate the ability of HIV-1 Tat to activate monocytes and microglia l...
Cell phenotypes were analyzed by flow cytometry. The following antibody clones were used: anti-CD3 (SK7, UCHT1), anti-CD56 (MY31, N901), anti-CD14 (MphiP9), anti-CD45RA (L48), anti-CD45RO (UCHL1), anti-CCR7 (150503), anti-CD4 (SK3) and anti-CD8 (T8). For the detection ...
the mice were either challenged with phosphate-buffered saline as mock control or with 5 × 106monocyte-derived dendritic cells (derived from the same donor) pulsed with human CMV viral lysate. Blood samples were drawn on day 7 to determine the cell counts of human CD45+cells. On day 14,...
Quantification of T-cell, B-cell, granulocyte, and monocyte recoveries by flow cytometry showed a rapid return to pretransplantation levels by day 35 (data not shown). Figure 4. MLCs and NK assays from 1 dog (H388) treated with 211At–anti-CD45 mAb-conditioned nonmyeloablative DLA-...
Briefly, cell aliquots were seeded in Iscove's modified Dulbeccco's medium containing 24% FCS, 1.3% methylcellulose, c-kit ligand (KL; 100 ng/ml), IL-3 (10 ng/ml), G-CSF (10 ng/ml), granulocyte-monocyte (GM)- CSF (10 ng/ml) (all gifts of the Kirin Brewing Co., Gunma, ...
We have avoided any manipulations such as carbonyl iron depletion or plastic adherence normally used for monocyte depletion because the abnormal B cells in MM are adherent and are depleted by these procedures. Normal B cells are not depleted and the procedure does not affect the intensity of ...
Effect of the monocyte chemoattractant protein-1/CC chemokine receptor 2 system on nephrin expression in streptozotocin-treated mice and human cultured podocytes. Diabetes. 2009;58:2109–18. Article PubMed CAS PubMed Central Google Scholar Bellini A, Mattoli S. The role of the fibrocyte, a ...
In some experiments, CD14+ monocytes were purified from PBMCs by directly conjugated anti-CD14 magnetic microbeads and magnetic cell separation (MACS) columns (both from Miltenyi Biotec, Surrey, United Kingdom) according to the manufacturer's instructions. Purity of the monocyte preparation was ...