Taken together, our observations indicate that ELCs can differentiate from cells of the monocytic lineage, suggesting a closer relationship between the monocyte/macrophage- and the endothelial cell systems than previously supposed.doi:10.1046/j.1432-0436.2000.6550287.xFernandez Pujol B...
This study monitored the circulating monocyte subsets and measured the plasma levels of IL-10 and IL-12 in response to H. pylori infection in 35 H. pylori-associated gastritis patients and 14 healthy controls. We found that the numbers of CD14(+)CD163(-)CD64(+) Ml-like monocytes as ...
人CD14+细胞阳性分选试剂盒用于阳性分选或者去除正常人外周血单个核细胞中的CD14+细胞,其利用生物素化抗体标记目的细胞,再用Anti-Biotin超顺磁纳米微珠结合被标记的目的细胞;当细胞悬液置于磁场中时,被磁珠结合的目的细胞滞留在磁场中,CD14-细胞悬浮在细胞悬液中,从而达到分选胡总恶化去除CD14+细胞的目的。
pylori(H.pylori)initiatesarobusthostimmuneresponseandsubsequentlyresultsinchronicin- ammationinthegastricmucosa.ThisstudymonitoredthecirculatingmonocytesubsetsandmeasuredtheplasmalevelsofIL-10andIL-12inresponsetoH.pyloriinfectionin35H.pylori-associatedgastritispatientsand14healthycontrols.WefoundthatthenumbersofCD14+CD...
Monocyte, dendritic cell, macrophage, endothelial cell, as well as smooth muscle cell markers were analyzed by real-time PCR, flow cytometry, and immunofluorescence staining. Results. In the LPS-treated group, most of suspended CD14-positive monocytes were attached and distinguishably changed in ...
Development of an ex vivo cellular model of rheumatoid arthritis: critical role of CD14- positive monocyte/macrophages in the development of pannus tissue. Arthritis Rheum 2007;56:2875-85.Nozaki T, Takahashi K, Ishii O, Endo S, Hioki K, Mori T, Kikukawa T, Boumpas DT, Ozaki S, Yamada ...
Both soluble factors and direct-cell contact interactions, such as JAG/NOTCH and COX-2/PGE2 pathways, are critically involved in the HSC-monocyte crosstalk. Our results indicate that the infusion of monocytes improves engraftment when cell dose is a limiting factor....
A propagation method of natural killer cells from the umbilical cord blood CD14 positive monocyte includes a step of mixing the umbilical cord blood CD14 positive monocyte with IL-15 and IL-21. The CD14 positive monocyte is manufactured by the following steps: manufacturing CD3-CD34-CD56-CD19 ...
It is a kind of to prepare the monocyte that langerhans cell is human peripheral from CD14-positive cells, including culture human peripheral blood mononuclear cell in the presence of recess ligand, GM-CSF and TGF-β.Naoyuki KatayamaKoshi Oishi...
Using monocyte-derived microparticles (MPs) released from human monocytes, incubated with or without lipopolysaccharides (LPS) in vitro, we measured MP-associated TF-protein (flow cytometry) and TF-activity (clot formation assay). MPs released from monocytes exposed to LPS (1 ng ...