Active tumor cell motility has long been appreciated to play a major role in invasion and metastasis. Tumor cells exhibit an amoeboid movement similar to that of polymorphonuclear (PMN) leukocytes and the lower eukaryote Dictyostelium , characterized by pseudopod protrusion at the leading edge of ...
Histological sections of tumors greater than 35 mm3 were stained with PAS, with CD-31 antibody (an endothelial cell maker), or with hypoxia inducible factor 1?? antibody (HIF). The extent of blood vessel and endothelial cell pseudopod volume density was measured by ocular grid intercept ...
is controversial and unknown. Most theories state that cell movement involves active changes in cell shape, brought about by forces generated by metabolic energy, such as pseudopod formation and withdrawal, but there is no agreement about the site and nature of the forces ...
MDA-MB-231 human breast cancer cells were used to study attachment and detachment during the cell cycle as well as explore the influences of the chemokine SDF-1 (enabling the quantification of the role of chemokine gradients both on pseudopod formation and directional cell migration). 展开 ...
PTEN plays a role in the suppression of lateral pseudopod formation during Dictyostelium motility and chemotaxis. J. Cell Sci. 120, 2517–2531 (2007). Article CAS PubMed Google Scholar Vicente-Manzanares, M., Zareno, J., Whitmore, L., Choi, C. K. & Horwitz, A. F. Regulation of ...
[8]. This provides a possible basis for the difference in the effects of SCAR phosphorylation on growth and endocytosis and pseudopod formation. If signals such as Rac activation directly cause actin polymerization during macropinocytosis, modulation of SCAR activity will have a limited effect—even ...
anionic but not cationic surfaces promoted two modes of pseudopod formation by HCS-2/8 cells, in a random progression and as a “cell recognition signal”. This phenomenon was associated with a cellular mechanism to optimize the anchoring process. Interestingly, cells developed pseudopods on cation...
In spite of these dramatic changes in pseudopodium behaviour, the underlying motility cycle of both mutants remains normal. The levels of F-actin in both mutant cell lines are significantly elevated with respect to the wild-type parental cells, suggesting a possible biochemical basis for these ...
Drug targets with genetic evidence are expected to increase clinical success by at least twofold. Yet, translating disease-associated genetic variants into functional knowledge remains a fundamental challenge of drug discovery. A key issue is that the va
AHNAK is a giant protein associated with pseudopod formation and is often seen in extracellular particles15,16,17. In fact, AHNAK protein was detected in the cytosol of dental pulp cells, but not Hek293 cells (Fig. 10a), and also in dental pulp tissue (Fig. 10b). In addition, AHNAK ...