Overview: assays for studying integrin- dependent cell adhesion. Methods Mol Biol. 2012;757:3e14.Chigaev A, Sklar LA. Overview: assays for studying integrin-dependent cell adhesion . Methods Mol Biol . 2012; 757 :3–14. Epub 2011/09/13. doi: 10.1007/978-1-61779-166-6_1 ....
Kim et al showed that the full-length TGFBIp and the four FAS1 domains adhere to MRC-5 fibroblasts through αvβ5integrin but not by α1β1.63Inhibitory assays suggested that the fourth FAS1 domain exhibited strong inhibitory effect (EC50= 5 – 10 μM) on the cell adhesion of full-leng...
It is important to realize that cell adhesion is a complex process that involves many different molecular interactions, including receptor–ligand binding, changes in the fluxes through intracellular signaling pathways, and modulation of cytoskeletal assembly. Consequently, adhesion assays not only measure ...
cell adhesionAtom RSS Feed Cell adhesion articles within Scientific Reports FeaturedArticle 31 October 2024 | Open Access TSPO deficiency promotes the progression of malignant peripheral sheath tumors by regulating the G2/M phase of the cell cycle via CDK1 Xingnan Zhang , Chenhao Hu & Song ...
cells.IndexEntries:Adhesion;integrin;spreading;attachment;extracellularmatrix.1.IntroductionThisarticlewilloutlineindetailthetwostan-dardassaysusedintheauthor’slaboratoryforquantitatingtheadhesionofcellstoanimmobi-lizedsubstrate.Theattachmentassay,whichemploysacolorimetricdetectionofboundcells,isbasedonKuengetal.(1),...
Cell adhesion makes an important contribution to the maintenance of tissue structure, the promotion of cell migration, and the transduction of information ... MJ Humphries - 《Molecular Biotechnology》 被引量: 240发表: 2001年 Cell adhesion assays Cell adhesion makes an important contribution to the...
aCell adhesion molecules integrin αV can influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells and the inhibition of cell apoptosis might be its mechanism, proved by three dimensional culture model to mimic solid tumor in vivo. 细胞粘着分子integrin αV可能影响人的鼻咽癌CNE-...
Thus, MAP kinases are activated when cells adhere to substrata coated with the integrin ligands fibronectin or laminin, but not when cells adhere to poly-D-lysine, a nonspecific adhesion-promoting polypeptide. Treatment of cells with cytochalasin D, an inhibitor of actin microfilament assembly, ...
However, glyphosate has not been reported previously to act as a ligand for integrins. To confirm that glyphosate behaves as a cell integrin ligand, we performed cell adhesion assays on RGD-displaying polymer (well-known to promote cell adhesion) and glyphosate surfaces using the Epic BT ...
Cell adhesion to III1-C results in robust ERK1/2 activation that is blocked by integrin-blocking agents. In addition, cell adhesion to III1-C and ERK1/2 activation by III1-C are both inhibited by heparan sulfate but not by chondroitin sulfate. Moreover, heparitinase treatment, but not ...