Rojas, "Modulatory effects of cd14+cd16++ monocytes on cd14++cd16- monocytes: a possible explanation of monocyte alterations in systemic lupus erythematosus," Arthritis and Rheumatology, vol. 66, no. 12, pp. 3371-3381, 2014.Burbano C, Vasquez G, Rojas M. Modulatory effects of CD14 + CD...
Hemodialysis patients (HDs) had significantly higher counts of CD14++CD16+ and CD14+CD16+ monocytes compared to patients on peritoneal dialysis (PD) treatment, whereas the difference in CD14++CD16− monocyte counts did not reach statistical significance (CD14++CD16− cells: HD 455±235 cells...
In peripheral blood the majority of circulating monocytes present a CD14highCD16− (CD14++) phenotype, while a subpopulation shows a CD14lowCD16+ (CD14+CD16+) surface expression. During haemodialysis (HD) using cellulosic membranes transient leukopenia occurs. In contrast, synthetic biocompatible ...
Peripheral blood was obtained from 143 patients suffering from PAOD (Rutherford stage I to VI) and three monocyte subsets were identified by flow cytometry: CD14++CD16− classical monocytes, CD14+CD16++ non-classical monocytes and CD14++CD16+ intermediate monocytes. Additionally the expression of ...
Based on the difference in the CD14 and CD16 expression, two subsets of monocytes were identified in human and other mammalian blood. These subsets have different patterns of adhesion molecules and chemokine receptors that suggests the different mode of their interaction with endothelium and tissue tr...
Human monocyte subsets were purified with MACS technology based on the differential CD14 and CD16 expression, yielding a purity of 98.0% ± 0.6% for CD14++CD16− monocytes, 89.3% ± 5.2% for CD14++CD16+ monocytes, and 96.3% ± 3.3% for CD14+CD16++ monocytes, with a mean viability of...
2014. The CD14+CD16+ inflammatory monocyte subset displays increased mitochondrial activity and effector function during acute Plasmodium vivax malaria. PLoS Pathog. 10: e1004393.Antonelli LR, Leoratti FM, Costa PA, Rocha BC, Diniz SQ, Tada MS, Pereira DB, Teixeira-Carvalho A, Golenbock DT, ...
The CD14(bright) CD16+ monocyte subset is expanded in rheumatoid arthritis and promotes expansion of the Th17 cell population. Arthritis Rheum. 2012;64(3):671-677.Rossol M, Kraus S, Pierer M, Baerwald C, Wagner U. The CD14(bright) CD16+ monocyte subset is expanded in rheumatoid arthritis...
(a) Flow analyses showing the purity of isolated monocyte subsets using anti-CD14 and anti-CD16 on a FACSCalibur. Percentages indicate purity of the subsets. Plots shown are representative of all isolations. Isolated monocytes are then used for transcriptomic and proteomics, and the flow diagram...
Based on the TST and IGRA tests, household contacts were divided into 'LTBI' and 'TB Resisters'. Peripheral blood mononuclear cells (PBMCs) of the study participants were isolated and processed to characterize the monocyte subsets based on CD14 and CD16 expression in flow cytometry. Monocyte ...