These cells constitutively express MHCII and the costimulatory molecules, CD80, CD86, and CD40. They do not express pan markers of myeloid DCs (CD11c), plasmacytoid DCs (Ly6C), or macrophages (F4/80), and their frequency is inversely correlated with myeloid‐derived suppressor cells (MDSCs) ...
Clinically Relevant Allergen Mixture Induces Robust Immune Response By Increasing CD11c+CD11b+MHCIIhiCD103int Lung Dendritic CellsFemale Balb/c mice were sensitized intranasally and challenged by aerosolizing a combination of house dust mite, ragweed and or PBS for a total of 5 weeks. Lung tissue...
These cells constitutively express MHCII and the costimulatory molecules, CD80, CD86, and CD40. They do not express pan markers of myeloid DCs (CD11c), plasmacytoid DCs (Ly6C), or macrophages (F4/80), and their frequency is inversely correlated with myeloid‐derived suppressor cells (MDSCs) ...
7. 小鼠肠道组织 肠道组织里,很多老师都会研究DC细胞,但是DC细胞的标志物CD11C,MHCII在肠道巨噬细胞中也会有表达,老师们在检测和数据分析圈门时要注意哦。 8. 小鼠皮肤组织 除了CD11B,F4/80marker外,可添加EPCAM,CD64,CD24等标...
. (c) Similar tob, CD11chiCD64−B220−DCs were purified from the lymph of egg-injected C57BL/6 or MHCII−/−MLNx donor mice, transferred into the MLNs of recipient animals and antigen specific T-cell responses measured afterin vitrorestimulation with SEA (n=6 mice per group, in...
Classical lymphoid organ-resident CD11chighMHCII þ CD8 À CD11b þ DC fraction was effectively diminished by 80% (Figure 2b). Interestingly, for unknown reasons, DT-treated CX3CR1GFP/Clec4a4-DTR mice, but not DT-treated CX3CR1GFP/Clec9A-DTR mice, also partially ablated the CD11c...
the SILP ofRag1−/−Cre−mice contained four subsets of bona fide CD11c+MHCII+CD64−DCs, distinguished by their differential expression of CD103 and CD11b (Fig.3aand Supplementary Fig.2a). The absolute numbers and proportions of DCs co-expressing CD11b and CD103 were severely reduced...
sFliC immunized mice had an increased frequency of CD11c þ MHC-IIhi cDCs in MLN (Figure 1a). In the steady state this population has been suggested to contain cDCs that have migrated from the SI-LP28 and potentially some resident CD8a þ and CD11b þ cDCs that have upregulated MHC...
(a) Flow cytometry analysis of CD11b þ and CD8a þ in CD11c þ and MHC II þ gated DCs in the spleen of naive mice or mice immunized intravenously (i.v.) with CT 24 h before analysis. (b) CD86 expression in CD11b þ or CD8a þ DCs in naive (gray line) or CT-...
Clinically Relevant Allergen Mixture Induces Robust Immune Response By Increasing CD11c+CD11b+MHCIIhiCD103int Lung Dendritic CellsSannette C. HallDevendra K. AgrawalElsevier BVJournal of Allergy and Clinical Immunology