A ChIP grade ETS1 antibody (Cell Signaling) was used for immunoprecipitation of ETS1, following the elution of ETS1-bound DNA. The amount of caspase-1 DNA was quantitated using RTqPCR using primers for the caspase-1 promoter (IDT). A non-specific IgG (Cell Signaling) was used as a ...
《Downregulation of circular RNA hsa_circ_0001649 indicates poor prognosis for retinoblastoma and regulates cell proliferation and apoptosis via AKT/mTOR signaling pathway》 作者:Lichen Xing,Leiming Zhang,Yali Feng,Zhe Cui,Lin Ding 期刊:Biomedicine & Pharmacotherapy 影响因子:3.743 PMID:29864621 ...
使用的抗体包括:caspase-1(clone4b4,由vishvam.dixit(美国genetech赠与)(kayagaki等人,2015),caspase-3(9662,cellsignaling),pro-caspase-8(4790,cellsignaling),剪切后的caspase-8(9429,cellsignaling),apaf1(8723,cellsignaling),caspase-9(9508,cellsignaling),gsmdd(ab209845,abcam),asc(67824,cellsignaling),...
24、Clerc P, Jeanjean P, Hallalli N, Gougeon M, Pipy B, Carrey J, Fourmy D, Gigoux V. Targeted Magnetic Intra-Lysosomal Hyperthermia produces lysosomal reactive oxygen species and causes Caspase-1 dependent cell death. J Control Release. 2017. Dec 1;270:120-134. doi: 10.1016/j.jconrel...
Western Blot (see online protocol):(1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation) Immunohistochemistry:(1:500; paraffin sections) Immunoprecipitation:(1:200) CrossreactivityMouse ...
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Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor. 仅用于科研。不用于诊断过程。未经明确授权不得转售。
Anti-GAPDH antibody (1:1000 dilution; Ab9485) was obtained from Abcam. Anti-IL-1β antibody (1:1000 dilution; catalog no. 2022) was purchased from Cell Signaling Technology. Human recombinant caspase-1 was from Merck. TRIzol RNA extraction reagent, oligo(dT)12–18 primers, random hexamers,...
Caspase 1 (cleaved Asp297) was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. Caspase 1 (cleaved Asp297) has been shown to induce cell apoptosis...
Neuronal caspase-3 signaling: not only cell death - 结果分析:横坐标为活化的Caspase-3强度,活化的Caspase-3阳性的细胞即为发生凋亡的细胞(红色部分)。 变化四:DNA片段化 细胞在凋亡时,染色质发生浓缩, 染色质DNA在核小体单位之间的连接处断裂, 形成180~200bp及其整数倍的寡核苷酸片段,。DNA一旦发生片段化,在...