Results In normal retina, no caspase-1 positive cells were seen. One hour after ischemia, numerous positive cells were noted in the ganglion cell layer (GCL) and inner side of inner nuclear layer (INL). At 3 hours, caspase-1 positive cells continued to increase and peaked at 6 hours, ...
Positive Controls:HL-60whole cell lysate:sc-2209,HL-60+LPS cell lysate:sc-24704or Jurkat whole cell lysate:sc-2204.RECOMMENDED SECONDARY REAGENTS To ensure optimal results,the following support (secondary)reagents are recommended:1)Western Blotting:use goat anti-mouse IgG-HRP:sc-2005(dilution ...
Our results show that CLP-induced hippocampus-dependent memory deficits are accompanied by increased NLRP3 and caspase-1 positive cells, and augmented protein levels of NLRP3, caspase-1, gasdermin-D, and pro-inflammatory cytokines in the hippocampus. In addition, administration of MCC950 or Ac-...
Caspase activation was quanti- fied by immunostaining for active caspase-3, using a cleaved caspase-3 antibody (Cell Signaling). Hepatocyte apoptosis in liver sections was quantitated by counting the number of TUNEL-positive or active caspase-3-positive cells in five random microscopic fields ( ...
4F). Meanwhile, the number of PI-positive cells and the release of LDH were reduced as well (Fig. 4G-I). The release of IL-1β and IL-18 were decreased in the cell supernatant after NAC treatment (Fig. 4J, K). The above results indicated that TNF-ɑ-induced pyroptosis was reversed...
B–E. Inflammation-related pyroptosis proteins p-Akt, NLRP3, caspase-1, and pore-forming protein GSDMD were significantly increased in groups OAG, OAL, and OAH, whereas in group OAM downregulation of positive staining was observed. Data are presented as the mean ± SEM. *p < 0.05 vs...
TUNEL staining was performed with fluorescein-dUTP for apoptotic cell nuclei and 4′,6-diamidino-2-phenylindole (DAPI) to stain all cell nuclei. apoptosis index (AI), the number of TUNEL-positive cells divided by the total cells per field, was examined. Each AI was assessed in 20 randomly ...
positive regulation of interleukin-1 alpha secretion positive regulation of interleukin-1 beta secretion regulation of inflammatory response mitochondrial depolarization membrane hyperpolarization pyroptosis cellular response to mechanical stimulus cellular response to organic substance programmed necrotic cell death ...
After washing steps, Cell Death Discovery (2017) 17012 Official journal of the Cell Death Differentiation Association the percentage of activated caspase-positive cells was determined using fluorescence microscopy (Nikon eclipse TS100). Western blotting and immunoprecipitation Mf4/4 cells were infected ...
1b). Consistent with the results above, the number of propidium iodide (PI)-positive cells was greatly increased in both BMDMs and J774 cells by 300 μM propofol (Fig. 1c). These results provide initial evidence that propofol induces macrophage cell death. We further explored whether ...