Virulence assay in Drosophila inoculated by pricking Flies were anaesthetized with FlyNap (Carolina) and pricked in the thorax using a needle (BD Ultra-Fine Nano Pen Needles 32 g 5/32 inch) that was loaded with the tested strain of P. aeruginosa by dipping in a bacterial suspension at ...
Consistent with the results of the biofilm formation assay, the increased mRNA levels of napA, norV, and fnr in the yfeR mutant were not affected by tyrosol treatment, as shown by RT‒qPCR experiments; however, these mRNA levels returned to wild-type levels upon the complementation of pyfeR...
Utvikling av real-time PCR assay for påvisning av Staphylococcus epidermidis (Master thesis): Norwegian University of Science and Technology, Trondheim; 2016. Van Diek FM, Albers CGM, Van Hooff ML, Meis JF, Goosen JHM. Low sensitivity of implant sonication when screening for infection in ...
Biofilm formation assay The qualitative assay for biofilm formation was per- formed in Congo Red Agar (CRA) method and tube method, described by [61]. The biofilm was visualized using a scanning electron microscope. Bioinformatics analyses of Arginine Decarboxylase (ADC) Since the knowledge of ...
16 Antibiotic Susceptibility Testing The isolates were tested for their antimicrobial susceptibilities, and minimum inhibitory concentrations were determined with the Sensititer (TREK Diagnostic Systems Inc) plate assay17 following the manufacturer’s protocol. The minimum inhibitory concentration values were ...
Metatranscriptome sequencing was performed as follows: paired-end libraries were prepared using the Zymo-Seq RiboFree™ Total RNA Library Kit (the protocol includes the universal rRNA depletion step as well). Sequencing was done on an Illumina NextSeq sequencer using the TG NextSeq® 500/550 ...
(all-against-all) contrast as implemented Biofilm formation assay We analyzed biofilm formation of 33 Roseobacter strains that are deposited at the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany) via a crystal violet (CV) assay according to the protocol of O'...
Modified Miller units were calculated using the formula described in the original assay. All assays were performed in triplicate. Tn mutagenesis. The mini-Tn10 delivery vector pNKTXI-SceI (47) was used for transposon mutagenesis. Cultures of V. vulnificus bearing a PbrpA lacZ reporter and E. ...
Single cell assay for monitoring mCherry and GFP fluorescence was carried out by time-lapse fluorescence microscopy using microfluidic plates. Overnight cultures in LB of S. aureus V329 Bap-mCherry and Bap-Spy were diluted to an initial OD600nm of 0.03 in LB and grown at 37 °C and 200...
Root colonization assay To determine the colony number of tomato root colonizing B. velezensis DMW1 and P. protegens Pf-5/ΔpltB in the rhizosphere soil, thirty-day-old tomato-plant seedling roots were dipped into the bacterial suspension containing 5 × 107 cells/mL Bacillus and 3 × 108 ...