Basic biofilm assay protocols that can be performed in any microbiology laboratory are reported along with the use of bacteriophages as biocontrol agents.doi:10.1007/978-1-0716-0607-0_7Pramila Devi UmraoVineet KumarSadhana Singh SagarShilpa Deshpande Kaistha...
Cell vitality assay kits DNA- and RNA-based cell stains Voltage and membrane potential sensors Biochemical, drug, and antibiotic conjugates LPS conjugates Organelle stains Other enzyme assay kits Related Articles Read more about biofilms in issue 59 of BioProbes® Journal. Download the...
The target protein was then detected using the eECL Western Blotting Kit (CoWin Biosciences, China) according to the manufacturer’s protocol. Microscale thermophoresis (MST) assay Microscale thermophoresis (MST) was performed to qualitatively analyze the interaction between BpfD-In and CRP. Proteins ...
The second strategy employed “post-treatment” exposure. Mature biofilms were first cultivated in a Calgary Biofilm Device (CBD; MBECTM Assay System, MBEC Biofilms Technology Ltd., Calgary, Alberta, Canada) for 7 days. Subsequently, they were exposed to the test compounds. This approach aimed t...
Crystal violet assay quantifying biofilm for- mation of a P. aeruginosa PAO1 and b P. protegens Pf5 when cell-free supernatants from various K. pneumoniae strains were added. Cell-free super- natants of a 12 h culture of K. pneumoniae were either added without any treatment (untreated CFS...
were separated according to the previously described method. The polysaccharides were determined using phenol‒sulfuric acid method as described above. Extracellular protein levels were quantified using the BCA Protein Assay Kit, following the manufacturer's protocol precisely. The bacterial residues in bi...
Crystal violet assay quantifying biofilm for- mation of a P. aeruginosa PAO1 and b P. protegens Pf5 when cell-free supernatants from various K. pneumoniae strains were added. Cell-free super- natants of a 12 h culture of K. pneumoniae were either added without any treatment (untreated CFS...
Utvikling av real-time PCR assay for påvisning av Staphylococcus epidermidis (Master thesis): Norwegian University of Science and Technology, Trondheim; 2016. Van Diek FM, Albers CGM, Van Hooff ML, Meis JF, Goosen JHM. Low sensitivity of implant sonication when screening for infection in ...
Enzyme activity inhibition assay SRH (the LuxS substrate) was prepared according to a previous protocol [32]. A 1 mg/mL solution of SAH and 1 M HCl was reacted in boiling water for 20 min. The SRH solution was diluted to 4 mM with 100 mM sodium phosphate buffer. The effects of natur...
Protein samples were precipitated by using the phenol protocol of Hanna et al. (2000). The protein concentration was determined by using the modified Bradford assay method (Ramagli, 1999). The protein samples were stored at −80° C. until proteomic analysis. 2-4. Two-Dimensional Gel Electr...