Consistent with the results of the biofilm formation assay, the increased mRNA levels of napA, norV, and fnr in the yfeR mutant were not affected by tyrosol treatment, as shown by RT‒qPCR experiments; however, these mRNA levels returned to wild-type levels upon the complementation of pyfeR...
3 Because of the formation of a spatial structure consisting of many components (polysaccharides, lipids, proteins, and extracellular DNA), EPS has a protective function against adverse environmental factors while at the same time creating a distinct habitat for bacteria anchored in the biofilm. ...
This suppression leads to decreased NO production, elevated bis-(3’−5’)-cyclic dimeric GMP levels, and finally stimulates anaerobic biofilm formation in the mature stage. Blocking YbfA with tyrosol treatment renders biofilm cells as susceptible to antibiotics as planktonic cells. Thus, this ...
PA, USA). Quantification was performed using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) on a Qubit 3.0 Fluorometer (Thermo Fisher Scientific) according to the manufacturer’s instructions. The
The biofilm formation capacity of the 100 A. baumannii clinical isolates in this study showed that 49%, 49%, and 2% of isolates were strong, moderate, and weak biofilm producers, respectively (Fig. 2). Fig. 2 Result of the biofilm formation assay using the microtiter plate test Full size...
The focus of this study was to investigate the effects of luxS, a key regulatory gene of the autoinducer-2 (AI-2) quorum sensing (QS) system, on the biofilm formation and biocontrol efficacy against Ralstonia solanacearum by Paenibacillus polymyxa HY96-2. luxS mutants were constructed and assa...
However, a higher significance of the “pre-treatment strategy” to modulate the biofilms was observed, that can be attributed to its impact on biofilm formation, confirmed by the much less co-expression with the respective control in the metatranscriptome analysis than for the post-treated ...
www.nature.com/scientificreports OPEN Low-level predation by lytic phage phiIPLA-RODI promotes biofilm formation and triggers received: 23 August 2016 accepted: 13 December 2016 Published: 19 January 2017 the stringent response in Staphylococcus aureus Lucía Fernández, Silvia González, Ana ...
(s) 2023 Abstract Objectives The present study aimed to evaluate the potential of the salivary pellicle (SP) formed on titanium (Ti) surfaces to modulate the formation of a biofilm composed of Streptococcus gordonii, Actinomyces naeslundii, Fusobacterium nuclea- tum, and Porphyromonas gingivalis...
16 Further, a dot blot assay showed that NGM significantly decreased the amount of PIA in the ECM of SH1000 and SE4 (Fig. 1e, Fig. S2a, c). The ability of NGM to inhibit biofilm formation by SH1000 and SE4 thus appears to be dependent on inhibition of PIA production. In contrast...