The results of the XTT reduction assay and crystal violet (CV) staining assay demonstrated that tea seed saponin monomers concentration-dependently suppressed the adhesion and biofilm formation of C. albicans and were able to eradicate mature biofilms. The compounds were in the following order in ...
specifically focusing on two AHL acylases enzymes; PvdQ and MacQ, to enhance their biochemical properties and kinetic efficiency. They employed a novel time-course kinetic assay to improve the activity of these acylases. For PvdQ, engineering efforts led to significant stabilization, with an incre...
Quantitative real-time PCR assay evaluated bap gene expression in bacteriocin-treated cells. According to SDS-PAGE, bacteriocin from L. plantarum has a 25-kDa apparent molecular weight. The MICs of bacteriocin ranged from 30 to 120 渭g/mL, while the MBCs varied between 60 and 120 渭g/mL. ...
after cleaning76. Over 20 passages in our assay, we observed no change in EPS inhibition (Fig.3aand Supplementary Fig.8). This was also reflected at the transcriptional level:csgDexpression in strains isolated from the last day of the evolution experiment is just as suppressed as in the pare...
According to Marova and Kovar, lysostaphin’s bacteriolytic activity was assessed using a spectrophotometric assay [71] with few modifications: The reaction mixture was preincubated at 37oC for 10 min with a suspension ofS. aureusATCC 29,213 cells in 0.1 M phosphate buffer (pH 7.5) to an OD...
2.4.1. Preliminary screening (Disk diffusion assay) To evaluate the anti QS capacity of the A. tsaoko extract, the qualitative disk diffusion method was used as the previously published procedure (Khan et al., 2009). An aliquot of 100 μl fresh C. violaceum culture dilution was plated on...
Biofilm production inhibition assay Biofilm inhibition by cranberry PACs was measured by the crystal violet stain method with some alterations [27]. P. aeruginosa ATCC 9027 (105 CFU) in TSB was incubated with varying concentrations of PACs, or without treatment, in triplicate in a 96-well plate...
performed antimicrobial assay. S.K.Z., F.G. and S.P.J. prepared the manuscript. Ethics declarations Competing interests The authors declare no competing financial interests. Electronic supplementary material Supplementary Information Rights and permissions This work is licensed under a Creative Commons ...
To further elucidate the bactericidal effect of VC on the two CR-hvKP strains, a time-kill curve assay was performed. The growth of KP1088 at both early (Figure 1A) and late exponential phases (Figure 1B) were found to be inhibited by VC at 8 mg/mL (MIC) within 12 h. Bacterial ce...
After the treatment, the biofilms were washed gently with 100 μL of PBS, and their vitalities were measured by a 2,3-bis (2-methoxy-4- nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay with some modifications.14 Then, 200 μL of freshly prepared reaction ...