An anti-biofilm assay was performed using a minimum biofilm inhibitory concentration test. Among herbs, only P. granatum showed an antibacterial effect. Therefore, a nanoliposomal formulation ofwas developed and characterized. Its effect onglucosyltransferases was assessed by measuring glucan amount. The...
after cleaning76. Over 20 passages in our assay, we observed no change in EPS inhibition (Fig.3aand Supplementary Fig.8). This was also reflected at the transcriptional level:csgDexpression in strains isolated from the last day of the evolution experiment is just as suppressed as in the pare...
specifically focusing on two AHL acylases enzymes; PvdQ and MacQ, to enhance their biochemical properties and kinetic efficiency. They employed a novel time-course kinetic assay to improve the activity of these acylases. For PvdQ, engineering efforts led to significant stabilization, with an incre...
According to Marova and Kovar, lysostaphin’s bacteriolytic activity was assessed using a spectrophotometric assay [71] with few modifications: The reaction mixture was preincubated at 37oC for 10 min with a suspension ofS. aureusATCC 29,213 cells in 0.1 M phosphate buffer (pH 7.5) to an OD...
In vitro biofilm assay experiments demonstrated that AgCl–TiO2 nanocomposite coated surfaces, inhibited the development of biofilms over a period of 10days as confirmed by scanning electron microscopy. The silver release kinetics exhibited an initial high release, followed by a slow and sustained ...
investigations were performed to determine their efficacies as QSI. Using a live reporter assay for QS, 5 compounds were demonstrated to be able to suppress QS-regulated gene expression inP. aeruginosain a dose-dependent manner and obviously regulate 46 proteins (19 were upregulated; 27 were ...
2.4.1. Preliminary screening (Disk diffusion assay) To evaluate the anti QS capacity of the A. tsaoko extract, the qualitative disk diffusion method was used as the previously published procedure (Khan et al., 2009). An aliquot of 100 μl fresh C. violaceum culture dilution was plated on...
the traditional crystal violet assay. Both biofilm assays showed that Ga(III)–ME0163 inhibited biofilm formation more efficiently at low concentrations than Ga(III)–citrate did, while ME0163 alone had very little effect on biofilm formation (Fig. 3a). Bacterial growth curves showed that Ga(III...
The assay was carried out using the previously described crystal violet protocol. Wells containing culture medium without AgNPs was used as the control. The percentage of biofilm inhibition was calculated using the formula [24]: $${\%} \;\text{Percentage inhibition }= [({\text{OD}}_{\text...
Moreover, the time-kill curve assay results indicated that the combination of drugs can effectively inhibit the planktonic S. epidermidis. After drugs treatment, the drug-combination showed significantly inhibitory effects on the metabolic activity and total biomass in each stage of biofilm formation. ...