Minimum biofilm eradication concentration (MBEC) assay The MBEC was measured using a microtiter plate-based technique. Briefly, 180 μL of MRSA LAC or PAO1 suspension (cell density at ~107 CFU/mL) in TSB was added to a 96-well plate covered by a lid containing pegs (Innovotech 19111)...
In MTT assay showed a greater cytotoxic effect on SW480 cancer cells at higher concentrations for longer periods of time. The present study showed that the cumin ethanolic crude extract has antimicrobial properties, inhibits the growth of S. mutans biofilms and has no toxi...
Furthermore, in vivo mice protection assay was conducted to attest the LAM's anti-pathogenic activity. The current findings elaborated the promising anti-pathogenic and anti-QS activities of LAM. LAM interfered with biofilm formation in P. aeruginosa PAO1 strain. Moreover, swarming motility and ...
removed by double washing with PBS and the dye remaining within viable cells was dissolved over 10 min (gentle shaking) using 1 mL of Neutral Red Assay Solubilization Solution (Sigma). The intensity of Neutral Red dye was measured spectrophotometrically at 540 nm using Fluoroskan plate reader (T...
The high-throughput ATAD5-luciferase assay in a 1536-well plate-based format was developed to identify compounds that enhance human ATAD5 protein levels, by creating an HEK293T cell line that could stably express luciferase-tagged ATAD5. The luminescence intensity of the assay plates was ...
(Invitrogen, Thermo Fisher Scientific Waltham, Massachusetts, USA) [41, when used in microtiter assay42, when used with confocal laser scanning microscopy (CLSM)] in the F-bottom black 96-well plate according to the manufacturer protocol (the fluorescence intensity was measured at a wavelength ...
The BODIPY-cadaverine assay indicated that the potent anti-inflammatory activity of Lf6-pP may be attributed to its direct interaction with LPS, resulting in decreased TNF-α and IL-6 levels in LPS-stimulated macrophages. Mechanistic studies, including membrane depolarization, outer/inner membrane ...
Quantification of reducing sugar was done through phenol–sulphuric acid assay following the protocol of Mecozzi38. Antibiofilm effect of CuO NPs Effect of NPs on biofilm formation by antibiotic resistant bacteria was also checked. Briefly, overnight bacterial cultures were standardized to 0.5 Mc...
The time kill analyses were performed using a modification of the broth microdilution assay42. The histone treatment is based on the minimum inhibitory concentration (MIC) required to prevent any visible increase in bacterial turbidity above baseline (absorbance). Briefly, planktonic cells (50 μl...
The lowest concentration of an antibacterial agent that could hinder the bacterial apparent growth following overnight incubation is known as the Minimum Inhibitory Concentration (MIC). NPs antibacterial activity was measured using broth microdilution assay following the protocol of Hayat et al.34. CuO ...