The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data....
5.3Crystal violet assay Crystal violet(CV) assays were performed according to the protocol outlined byAlnuaimi et al. (2013).CVin a cationic dye that can bind to theextracellular polymeric substances(EPS) and the microorganisms that make up the biofilm matrix and stain the entire biofilm, makin...
用途:酸碱指示剂,pH0.5(绿)～pH 2.0(蓝); 非水溶液滴定指示剂;生物染色。 CAS编号:548-62-9 包装规格:25g 品牌:solarbio 货号:C8470 产地:北京 纯度:1 mg/mL in water% 是否进口:否 产品简介 1.中文名:结晶紫 2.英文名:Crystal violet 3.别 名:甲基紫10B;甲紫;六甲基紫;六甲基玫苯胺盐酸盐 ...
CN104818315A discloses a method for detecting the biofilm of Mycobacterium tuberculosis using Leucocrystal Violet staining. The method includes: Washing the biofilm; drying at 50 degrees Celsius; incubating in a 0.5-1.5% Leucocrystal Violet solution; washing and drying at 50 degrees Celsius; incubati...
rhamnosus GG SpaCBA pilus is reported to display a number of properties, which includes the adherence to mucus11,18,19, collagen protein20, and gut epithelial cells21, but as well, the stimulation of biofilm growth21 and various host-cell immune responses21–25. Undoubtedly, this type of ...
It indirectly determines the amount of biofilm by measuring the optical density (OD) of the crystal violet﹕tained biofilm matrix and cells. However, this test is quite timeヽonsuming, as it requires bacterial cultivation up to several days. In this study, we correlate fast Raman spectroscopic ...
aureus biofilm formation, the crystal violet (CV) assay and the XTT (tetrazolium salt reduction) assay, were optimized, evaluated, and further compared. In CV assay, most isolates formed weak biofilm 74.3 %), while the rest formed moderate biofilm (23.3 %) or strong biofilm (2.3 ...
Crystal violetBiofilmSpectrophotometerPrecise quantification of biofilm is critical as the formation and persistence of biofilm have significant implications in the environmental, therapeutic and industrial contexts. The microtiter plate assay using crystal violet with 33% glacial acetic acid or 94–100% ...
Biofilm formation on abiotic surfaces observed in borosilicate glass tubes with crystal violet (A) and in glass slides with acridin orange (B, bar 500 μm).José David FloresFélixLuís R. SilvaLina P. RiveraMarta MarcosGarcíaPau...
Therefore, we focused on the staining method that used a crystal violet solution. In the previous study, we devised an evaluation process for biofilms using a color meter to analyze the various staining situations. However, this method was complicated and expensive for practical engineers. ...