(2)ATG16L1蛋白水平在AG和IG组的表达均低于HC组,P均<0.05,在AG组的表达低于IG组,P<0.05.(3)ATG12蛋白水平在AG组和IG组的表达低于HC组,P均<0.05,AG组和IG组之间差异无统计学意义,P>0.05.(4)ATG5蛋白水平在AG组的表达低于IG组和HC组,P均<0.05,IG组和HC组之间差异无统计学意义,P>0.05.(5)LC3B蛋白...
► Atg5-Atg12/Atg16L1 and Atg7 are required for IFNγ to inhibit norovirus replication ► The degradative activity of autophagy is not required for the antiviral activity of IFNγ ► IFNγ, via autophagy proteins, inhibits the formation of the replication complex ...
This conjugation switch is tightly regulated by ATG16L1, which binds to free ATG5 and mutually protects both proteins from ubiquitin conjugation and proteasomal degradation, thereby instead promoting the irreversible conjugation of ATG12 to ATG5. The resulting ATG12–ATG5 conjugate, in turn, displays ...
Hwang S et al (2012) Nondegradative role of Atg5- Atg12/Atg16L1 autophagy protein complex in anti- viral activity of interferon gamma. Cell Host Microbe 11:397-409Hwang, S., N. S. Maloney, M. W. Bruinsma, G. Goel, E. Duan, L. Zhang, B. Shrestha, M. S. Diamond, A. Dani...
Formation of autophagosomes, which mediate delivery of cytoplasmic cargo to lysosomes, requires multiple autophagy-related (ATG) protein complexes, including the ATG12–ATG5-ATG16L1 complex. Herein, we report that a molecular ATG5 "conjugation switch", comprised of competing ATG12 and ubiquitin ...
autophagyendosomeGABARAPLAPLC3lipidationmembraneThe machinery that decorates autophagic membranes with lipid-conjugated LC3/GABARAP is not yet fully understood. We recently reported the purification of the full-length ATG12鈥揂TG5-ATG16L1 complex, and in reconstitution experiments with purified ATG7, ATG3,...
Differences found within LC3/GABARAP proteins suggested the existence of a lipidation threshold as a requisite for tethering and inter-vesicular lipid mixing. The presence of ATG12-ATG5-ATG16L1 (E3 in short) increased and accelerated LC3/GABARAP lipidation and subsequent vesicle tethering. However, ...
GastroenterologyZhang H, Massey DC, and Parkes M. (2014). Expression as- sociation analysis of the ATG5, ATG12 and ATG16L1 genes in Crohn's disease (CD) (2014). Gastroenterology 146, S- 876. DOI: 10.1016/S0016-5085(14)63187-5
Vesicle tethering and fusion promoted by LC3/GABARAP proteins is modulated by the ATG12–ATG5-ATG16L1 complexRecently, we have examined the membrane anchoring and subsequent lipidation of six members of the LC3/GABARAP protein family, together with their ability to promote membrane tethering and ...
ATG5 is an essential autophagy related protein that acts as an E3-like activating enzyme of ubiquitin-like conjugating system in conjunction with ATG12 and ATG16L1. The ATG12鈥揂TG5/ATG16L1 complex is an essential complex which is crucial for autophagosome biogenesis. In this work, we have ...