This study aimed to identify SNPs in ATG5, ATG10, ATG12, and ATG16L1 associated with gastric mucosal atrophy (GMA). Here, two-hundred H. pylori-positive participants without gastric cancer were included. The degree of GMA was evaluated via the pepsinogen method. Twenty-five SNPs located in ...
Autophagy is critical for maintaining cellular homeostasis during times of stress, and is thought to play important roles in both tumorigenesis and tumor cell survival. Formation of autophagosomes, which mediate delivery of cytoplasmic cargo to lysosomes
aImmunoblot analysis of ATG12 protein inSETD2-deficient CAKI-1 cells andSETD2-competent ACHN cells, reveal the accumulation of both free ATG12 and of an additional ATG12-containing complex, distinct from the ATG5–ATG12 complex, in theSETD2-deficient RCC cells.bImmunofluorescence analysis show...
We have identified TECPR1 as a binding partner of the Atg12-Atg5 conjugate in an independent study. We show that TECPR1 and Atg16 exist in two mutually exclusive complexes with the Atg12-Atg5 conjugate. TECPR1 resides on lysosomes and recruits Atg12-Atg5 to autolysosomes. Remarkably, we ...
Yuan Gao1, Yanquan Wei1, Xiangtao Liu1, Ying Liao*,3, Huichen Guo*,1 and Shiqi Sun*,1 Autophagy-related protein ATG5-ATG12 is an essential complex for the autophagophore elongation in autophagy, which has been reported to be involved in foot-and-mouth disease virus (FMDV) replication...
Autophagy-related protein ATG5-ATG12 is an essential complex for the autophagophore elongation in autophagy, which has been reported to be involved in foot-and-mouth disease virus (FMDV) replication. Previous reports show that ATG5-ATG12 positively or ne
TECPR1ATG16L1 is an essential component of the Atg8-family protein conjugation machinery, providing membrane targeting for the ATG12-ATG5 conjugate. Recently, we identified an alternative E3-like complex that functions independently of ATG16L1. This complex utilizes the autophagosome-lysosome tethering...
The ATG12- ATG5-ATG16L1 or E3-like complex (E3 for short) acts as a ubiquitin-like E3 enzyme, promoting LC3/GABARAP proteins anchoring to the AP membrane. Their role in the AP expansion process is still unclear, in part because there are no studies comparing six LC3/GABARAP family ...
Nondegradative role of Atg5-Atg12/Atg16L1 autophagy protein complex in antivi- ral activity of interferon gamma. Cell Host Microbe 2012; 11: 397 - 409.Hwang, S., N.S. Maloney, M.W. Bruinsma, G. Goel, E. Duan, L. Zhang, B. Shrestha, M.S. Diamond, A...
The execution of canonical autophagy is regulated by a core signaling cascade and it involves two well-characterized, ubiquitin-like conjugation systems—the Atg5 / Atg12 / Atg16L1 and the Atg8 -phosphatidyl ethanolamine (PE), which are both catalyzed by Atg7 . The conjugation of Atg5 – Atg...