The Atg12-Atg5 conjugate promotes the transfer of Atg8 from Atg3 to the substrate, phosphatidylethanolamine (PE), by stimulating the activity of Atg3. We also show that the Atg12-Atg5 conjugate interacts with both Atg3 and PE-containing liposomes. These results indicate that ...
The Atg12-Atg5-Atg16 complex interacts directly with Atg3 via Atg12, and enhances the conjugase activity of Atg3 by rearranging its catalytic center, while it is targeted to the membranes via Atg5 and Atg16, and promotes the transfer of Atg8 from Atg3 to the membranes....
the catalytic cysteine residue of Atg3 is arranged in the catalytic center such that the conjugase activity is suppressed; Atg12–Atg5 induces a reorientation of the cysteine residue toward the threonine residue, which enhances the conjugase activity of Atg3. Thus, this study reveals the mechanism...
The resulting ATG12–ATG5 conjugate, in turn, displays enhanced affinity for ATG16L1 and thus fully stabilizes the ATG12–ATG5-ATG16L1 complex. Most importantly, we find in multiple tumor types that ATG5 somatic mutations and alternative mRNA splicing specifically disrupt the ATG16L1-binding ...
ATG12 increased cell apoptosis of HepG2.2.15 cells but not HepG2 cells under starvation conditions. These results suggest that ATG5-ATG12 proteins are important for the survival of HBV-associated HCC during states of limited tumor nutrients. The inhibition of ATG12 might be a good target for ...
ATG5-ATG12 Complex antibody 英文别名: ATG5-ATG12 Complex antibody 中文名: 中文别名: CBNumber: CB85606638 分子式: 分子量: 0 MOL File: Mol file 化学性质安全信息用途供应商6 ATG5-ATG12 Complex antibody化学性质 安全信息 ATG5-ATG12 Complex antibody性质、用途与生产工艺 ...
Remarkably, we demonstrate that TECPR1 plays a crucial function in autophagosome maturation and promotes autophagosome-lysosome fusion by associating with both the Atg12-Atg5 conjugate and PtdIns(3)P. Through this study, we have uncovered a previously unknown critical function of the Atg12-Atg5-TEC...
Yuan Gao1, Yanquan Wei1, Xiangtao Liu1, Ying Liao*,3, Huichen Guo*,1 and Shiqi Sun*,1 Autophagy-related protein ATG5-ATG12 is an essential complex for the autophagophore elongation in autophagy, which has been reported to be involved in foot-and-mouth disease virus (FMDV) replication...
In contrast, subsequent autophagosome maturation and closure events were unnecessary for HBV replication, as evidenced by inhibition of Atg8/LC3 conjugation. Interfering the HBV/Atg12 cross-talk may be a tool for virus control. 展开 关键词: Atg12-Atg5/Atg16L1 complex Atg8/LC3 conjugation ...
IHCplus Polyclonal Rabbit antiMouse ATG5ATG12 Complex Antibody (aa262275, IHC, WB) LSB9976| 基本信息 更多信息 中文名称: 中文同义词: 英文名称: IHCplus Polyclonal Rabbit antiMouse ATG5ATG12 Complex Antibody (aa262275, IHC, WB) LSB9976 英文同义词: IHCplus Polyclonal Rabbit antiMouse ATG5ATG...