Formation of the Atg12-Atg5-Atg16 complex is essential for its E3-like function: facilitation of Atg8 transfer from Atg3 to phosphatidylethanolamine at autophagic membranes. Structural studies on the Atg12-Atg5-Atg16 complex revealed that the unique architecture of this protein complex is totally ...
Toward the function of mammalian ATG12–ATG5-ATG16L1 complex in autophagy and related processesATG16L1Amphipathic helixGABARAPLAPLC3autophagyendosomelipidationmembraneThe machinery that decorates autophagic membranes with lipid-conjugated LC3/GABARAP is not yet fully understood. We recently reported the ...
While ATG16L2 stabilizes ATG5 and enables ATG12–ATG5 conjugation, this endogenous dominant-negative inhibitor simultaneously displaces ATG16L1, resulting in its proteasomal degradation and a block in autophagy. Thus, collectively, our findings provide novel insights into ATG12–ATG5-ATG16L1 complex ...
1.Nondegradative role of Atg5-Atg12/ Atg16L1 autophagy protein complex in antiviral activity of interferon gamma. and spring 机译:Atg5-Atg12 / Atg16L1自噬蛋白复合物在干扰素γ的抗病毒活性中的非降解作用。 Hwang S ,Maloney NS ,Bruinsma MW - Cell Host & Microbe - 2012 2.The roles of in...
Differences found within LC3/GABARAP proteins suggested the existence of a lipidation threshold as a requisite for tethering and inter-vesicular lipid mixing. The presence of ATG12-ATG5-ATG16L1 (E3 in short) increased and accelerated LC3/GABARAP lipidation and subsequent vesicle tethering. However, ...
Formation of autophagosomes, which mediate delivery of cytoplasmic cargo to lysosomes, requires multiple autophagy-related (ATG) protein complexes, including the ATG12–ATG5-ATG16L1 complex. Herein, we report that a molecular ATG5 "conjugation switch", comprised of competing ATG12 and ubiquitin ...
Atg18a is required for the PAS targeting of the Atg12–Atg5·Atg16 complex.LingLing, SunMing, LiFang, SuoXiaoMan, LiuEnZhi, ShenBing, YangMengQiu, DongWanZhong, HeLiLin, Du
Evodiamine inhibits the interaction of Atg5-Atg12 and Atg16 determined by the Co-immunoprecipitation assay.JianPing DaiWeiZhong LiXiangFeng ZhaoGeFei WangJiaCai YangLin ZhangXiaoXuan ChenYanXuan XuKangSheng Li
Mechanism and functions of membrane binding by the Atg5-Atg12/Atg16 complex during autophagosome formation. The EMBO Journal. 2012; 31 :4304–4317. doi: 10.1038/emboj.2012.278. [ Cross Ref ]Romanov J, Walczak M, Ibiricu I, et al. Mechanism and functions of membrane binding by the Atg5-...
Architecture of the Atg12–Atg5–Atg16 Complex and its Molecular Role in Autophagydoi:10.1016/B978-0-12-405529-2.00003-2Nobuo N. NodaFuyuhiko InagakiAutophagy: Cancer, Other Pathologies, Inflammation, Immunity, Infection, and Aging