Atg12结合系统泛素样蛋白Atg12跟其它的泛素样蛋白不同,C端结合有氨基乙酸所以无法进行RNA加工。E1是与Atg8共通的泛素转移酶Atg7。被传递给E2的泛素转移酶Atg10后最终被转递到Atg5。这个Atg12-Atg5的共价结合体与Atg16形成复合物。由于Atg16含有一个螺旋卷曲结构易形成底聚物,使其能把更多的Atg12-Atg5连接起来形成...
Formation of the Atg12-Atg5-Atg16 complex is essential for its E3-like function: facilitation of Atg8 transfer from Atg3 to phosphatidylethanolamine at autophagic membranes. Structural studies on the Atg12-Atg5-Atg16 complex revealed that the unique architecture of this protein complex is totally ...
atg12-atg5复合物又进一步与atg16结合形成atg12-atg5-atg16复合物。与atg16结合后将促进暴露位于atg5上的膜结合位点,从而有助于复合物与膜结合。研究证明,atg12-atg5-atg16复合物具有e3样酶生物学活性,主要通过激活atg3酶活性,促进atg8从atg3转移到底物磷脂酰乙醇胺(pe)上,进而完成自噬关键环节。 自噬可选择性降...
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自噬相关蛋白ATG12是ATG12基因编码的一种蛋白。在哺乳动物细胞中ATG12与ATG5形成复合物,在自噬的形成中发挥至关的重要。研究表明,自噬相关蛋白,如Beclinl,参与宫颈癌的发生、发展,在宫颈癌的发生发展中发挥重要的作用。然而ATG12在宫颈癌及癌前病变中的作用尚不明确。本研究的目的:(1)检测ATG12在正常宫颈上皮、...
Herein, we report that a molecular ATG5 “conjugation switch”, comprised of competing ATG12 and ubiquitin conjugation reactions, integrates ATG12–ATG5-ATG16L1 complex assembly with protein quality control of its otherwise highly unstable subunits. This conjugation switch is tightly regulated by ATG16...
ubiquitin-like conjugation systems the Atg12 and LC3-II systems. In the Atg12 conjugation system the Atg12-Atg5-Atg16L forms 800 kDa complex that elongates autophagic isolation membrane. After completion of the formation of the autophagosome the Atg12-Atg5-Atg16L complex dissociates from the ...
showing a reduction of LC3-II in autophagosomes.cWestern blot analysis was used to evaluate the expression of LC3 and p62.dSuppression of autophagy by knockdown of autophagy-related gene ATG5 could inhibit invasion of osteosarcoma cells.eOsteosarcoma cells treated with autophagy inhibitor 3MA also ...
Invitrogen Anti-ATG12-ATG5 Recombinant Monoclonal (20H20L20), Catalog # 702433. Tested in Western Blot (WB) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µg purified antibody (0.5 mg/mL).
ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd. Protocols Product Specific Protocols WB protocol for ATG12 antibody 11122-1-APDownload protocol ...