ATG16L1autophagyendosomeGABARAPLAPLC3lipidationmembraneThe machinery that decorates autophagic membranes with lipid-conjugated LC3/GABARAP is not yet fully understood. We recently reported the purification of the full-length ATG12鈥揂TG5-ATG16L1 complex, and in reconstitution experiments with purified ATG7...
This conjugation switch is tightly regulated by ATG16L1, which binds to free ATG5 and mutually protects both proteins from ubiquitin conjugation and proteasomal degradation, thereby instead promoting the irreversible conjugation of ATG12 to ATG5. The resulting ATG12–ATG5 conjugate, in turn, displays ...
1.Nondegradative role of Atg5-Atg12/ Atg16L1 autophagy protein complex in antiviral activity of interferon gamma. and spring 机译:Atg5-Atg12 / Atg16L1自噬蛋白复合物在干扰素γ的抗病毒活性中的非降解作用。 Hwang S ,Maloney NS ,Bruinsma MW - Cell Host & Microbe - 2012 2.The roles of in...
We recently reported the purification of the full-length ATG12-ATG5-ATG16L1 complex, and in reconstitution experiments with purified ATG7, ATG3, and LC3/GABARAP in vitro, together with rescue experiments in knockout cells, important aspects of the complete lipidation reaction were revealed. Hitherto...
Addition of a Sirt1 inhibitor following DJ-1 interference enhances the effect of DJ-1 interference on microglial polarization, decreases the level of the Atg5-Atg12-Atg16L1 complex, and inhibits autophagy. Conclusion:These results suggest that DJ-1 regulates the polarization of microglia during ...
Formation of autophagosomes, which mediate delivery of cytoplasmic cargo to lysosomes, requires multiple autophagy-related (ATG) protein complexes, including the ATG12–ATG5-ATG16L1 complex. Herein, we report that a molecular ATG5 "conjugation switch", comprised of competing ATG12 and ubiquitin ...
GastroenterologyZhang H, Massey DC, and Parkes M. (2014). Expression as- sociation analysis of the ATG5, ATG12 and ATG16L1 genes in Crohn's disease (CD) (2014). Gastroenterology 146, S- 876. DOI: 10.1016/S0016-5085(14)63187-5
Differences found within LC3/GABARAP proteins suggested the existence of a lipidation threshold as a requisite for tethering and inter-vesicular lipid mixing. The presence of ATG12-ATG5-ATG16L1 (E3 in short) increased and accelerated LC3/GABARAP lipidation and subsequent vesicle tethering. However, ...
Vesicle tethering and fusion promoted by LC3/GABARAP proteins is modulated by the ATG12–ATG5-ATG16L1 complexRecently, we have examined the membrane anchoring and subsequent lipidation of six members of the LC3/GABARAP protein family, together with their ability to promote membrane tethering and ...
ATG5 is an essential autophagy related protein that acts as an E3-like activating enzyme of ubiquitin-like conjugating system in conjunction with ATG12 and ATG16L1. The ATG12鈥揂TG5/ATG16L1 complex is an essential complex which is crucial for autophagosome biogenesis. In this work, we have ...