txci-ATAC-seq generates high-quality single-cell ATAC libraries at high throughput.aSchematic of molecular details of txci-ATAC-seq library generation.bExperimental workflow for txci-ATAC-seq barnyard library generation. After 96-plex tagmentation, nuclei are overloaded on a 10X Chromium microfluidics...
Usually, the first step for analysis of scATAC-seq data is the detection of OC regions by calling peaks on the scATAC-seq library by ignoring cell information. Next, a matrix is built by counting the number of digestion events per cell in each of the previously detected regions. This matrix...
refer to the SureCell ATAC-Seq Library Prep kit user guide (17004620, Bio-Rad). Collected cells and tagmentation buffers were chilled on ice. Lysis was performed simultaneously with tagmentation. After washing, equal numbers of cells from each donor were mixed with Whole-Cell Tagmentation Mix con...