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Cell surface antigen CD109 is a novel member of the α2 macroglobulin/C3, C4, C5 family of thioester-containing proteins. Blood 99, 1683–1691 (2002). Article CAS PubMed Google Scholar Bizet, A. A. et al. The TGF-β co-receptor, CD109, promotes internalization and degradation of TGF...
In the spatial sliding column, cells were bound to the S100β antibody (# 9550 S, Cell Signaling Technology, Danvers, MA, USA). Through negative selection, non-stellar glial cells were removed while stellar glial cells were retained. Subsequent washing steps were performed to eliminate cells and...
Figure 2. LacCer Boosts NF-κB-Driven Inflammation via cPLA2 Activation (A) Effect of LacCer on the enzymatic activity of recombinant human cPLA2 in a cell-free assay (unpaired t test, compared to control). (B) Pla2g4a expression in astrocytes 51 days after NOD EAE induction, and PLA2G...
When Tetrahymena pyriformis, strain NT-1, was warmed from a growth temperature of 15 degrees C to 39 degrees C, there was a rapid conversion of its sphingolipids from the nonhydroxy fatty acid-containing ceramide aminoethylphosphonate (NCAEP) predominant at low temperature to the alpha-hydroxy ...
cell, individual prion strains selectively recruit those sialoglycoforms that can be accommodated within a strain-specific structure [17,18,19]. As a result of selective recruitment, unique strain-specific patterns of carbohydrate epitopes is formed on PrPScsurface dictating strain-specific diseases ...
Three sets of analysis were performed: (1) 7 GFP+astrocyte samples were compared to 3 GFP−cell samples from the WT-GFP group, to validate sorting efficiency; (2) 4 GFP+astrocyte samples from APP-GFP mice were compared to 7 GFP+astrocyte samples from WT-GFP mice to identify transcription...
Using a cell- and region-specific approach2–4, we showed regional differences in astrocyte gene expression among CNS regions in the experimental autoimmune encephalomyelitis (EAE) mouse model of MS1. We found that cholesterol synthesis pathways were significantly downregulated in white matter rich ...
cell isolation steps for deep RNA sequencing, controlling the purity of the preparation.cHeat map illustrating differentially-expressed genes (False discovery rate, FDR < 0.05) between primary astrocytes from control andHmgb1ΔAstromice (n = 4 group).d,eVolcano plots (FDR versus Log2 ...
K. Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors. J. Biol. Chem. 288, 4209–4225 (2013). CAS PubMed Google Scholar Hartley, C. L. et al. Armet/Manf and Creld2 are components of a specialized ER stress ...