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Cell surface antigen CD109 is a novel member of the α2 macroglobulin/C3, C4, C5 family of thioester-containing proteins. Blood 99, 1683–1691 (2002). Article CAS PubMed Google Scholar Bizet, A. A. et al. The TGF-β co-receptor, CD109, promotes internalization and degradation of TGF...
and the top 10 gene names in highly variable genes. (B) Distribution of cells on PC_1 and PC_2, with each point representing a cell. (C) Distribution of standard deviation of PCs, with important
(A) Effect of LacCer on the enzymatic activity of recombinant human cPLA2 in a cell-free assay (unpaired t test, compared to control). (B) Pla2g4a expression in astrocytes 51 days after NOD EAE induction, and PLA2G4A expression in CNS samples from MS patients and controls; the same se...
When Tetrahymena pyriformis, strain NT-1, was warmed from a growth temperature of 15 degrees C to 39 degrees C, there was a rapid conversion of its sphingolipids from the nonhydroxy fatty acid-containing ceramide aminoethylphosphonate (NCAEP) predominant at low temperature to the alpha-hydroxy ...
Cell viability assay An MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed according to the manufacturer protocol. Primary neuronal cells were seeded in PDL-laminin coated 96-well culture plates at a density of 1 × 104cells per well in triplicates an...
Using a cell- and region-specific approach2–4, we showed regional differences in astrocyte gene expression among CNS regions in the experimental autoimmune encephalomyelitis (EAE) mouse model of MS1. We found that cholesterol synthesis pathways were significantly downregulated in white matter rich ...
Three sets of analysis were performed: (1) 7 GFP+astrocyte samples were compared to 3 GFP−cell samples from the WT-GFP group, to validate sorting efficiency; (2) 4 GFP+astrocyte samples from APP-GFP mice were compared to 7 GFP+astrocyte samples from WT-GFP mice to identify transcription...
cell isolation steps for deep RNA sequencing, controlling the purity of the preparation.cHeat map illustrating differentially-expressed genes (False discovery rate, FDR < 0.05) between primary astrocytes from control andHmgb1ΔAstromice (n = 4 group).d,eVolcano plots (FDR versus Log2 ...
K. Mesencephalic astrocyte-derived neurotrophic factor (MANF) secretion and cell surface binding are modulated by KDEL receptors. J. Biol. Chem. 288, 4209–4225 (2013). CAS PubMed Google Scholar Hartley, C. L. et al. Armet/Manf and Creld2 are components of a specialized ER stress ...