pathways related to mitosis and cell cycle checkpoints were also enhanced in WT-stimulated cells (Supplementary Tables1and2). This trend, which also occurs in reactive astrocytes35, is in line with evidence implicating Orai1 in cell proliferation20,36. Taken together, the results of the RNA-se...
Cell Surface Antigens of Human Astrocytoma Defined by Mouse Monoclonal Antibodies: Identification of Astrocytoma Subsets The surface antigens of cultured human malignant astrocytomas were analyzed by using mouse monoclonal antibodies. BALB/c mice were immunized repeatedly wit... JG Cairncross,MJ Mattes,...
Astrocytes and neurons extensively interact in the brain. Identifying astrocyte and neuron proteomes is essential for elucidating the protein networks that dictate their respective contributions to physiology and disease. Here we used cell-specific and s
at 1000 rpm. The obtained pellets were re-suspended in cold DPBS and cell suspension was subjected to debris removal and red blood cell removal. The single cell-suspension obtained was then subjected to magnetic labeling with an Anti-ACSA-2 MicroBead Kit (cat. 130-097-678, Miltenyi Biotec) ...
et al. Smart-seq2 for sensitive full-length transcriptome profiling in single cells. Nat. Methods 10, 1096–1098 (2013). Article PubMed CAS Google Scholar Macosko, E. Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 161, 1202–...
When Tetrahymena pyriformis, strain NT-1, was warmed from a growth temperature of 15 degrees C to 39 degrees C, there was a rapid conversion of its sphingolipids from the nonhydroxy fatty acid-containing ceramide aminoethylphosphonate (NCAEP) predominant at low temperature to the alpha-hydroxy ...
(MHC-I) and antigen-presentation pathway, which are restored by inhibiting Cdk5 genetically or pharmacologically, as evidenced by single-cell RNA sequencing and functional studies. Mechanistically, Cdk5 suppresses MHC-I expression on the cancer cell membrane through the Irf2bp1–Stat1–importin α–...
Three sets of analysis were performed: (1) 7 GFP+astrocyte samples were compared to 3 GFP−cell samples from the WT-GFP group, to validate sorting efficiency; (2) 4 GFP+astrocyte samples from APP-GFP mice were compared to 7 GFP+astrocyte samples from WT-GFP mice to identify transcription...
This was accompanied by an attenuated response to induction in long-term potentiation (LTP), suggesting that the hippocampal circuitry was perturbed upon hypoxic exposure following HIF-2α loss in astrocytes, and not due to hippocampal cell death. We investigated HIF-regulated trophic and metabolic ...
Cell surface antigen CD109 is a novel member of the α2 macroglobulin/C3, C4, C5 family of thioester-containing proteins. Blood 99, 1683–1691 (2002). Article CAS PubMed Google Scholar Bizet, A. A. et al. The TGF-β co-receptor, CD109, promotes internalization and degradation of TGF...