After incubation, we depleted myelin debris by 30% isotonic Percoll (P1644; Sigma-Aldrich) gradient centrifugation and obtained mononuclear cell suspensions in DMEM/F12 medium with ice-cold 10% heat-inactivated fetal bovine serum. We prepared unstained controls from mixtures of different sample cell ...
In particular, mouse models carrying human APOE4 have accelerated Aβ seeding and suppressed Aβ clearance, as well as disturbed synaptic plasticity and blood–brain barrier integrity7,9; however, whether these findings are translatable to humans is not clear. In addition, studies with human ...
com 【 Abstract】 The apolipoprotein E ( ApoE) is involved in cholesterol and lipid metabolism in the brain, transporting cholesterol to neurons and removing lipid debris to facilitate myelin repair. ApoE4, one of three subtypes of ApoE, is the most important genetic risk factor for sporadic ...
com 【 Abstract】 The apolipoprotein E ( ApoE) is involved in cholesterol and lipid metabolism in the brain, transporting cholesterol to neurons and removing lipid debris to facilitate myelin repair. ApoE4, one of three subtypes of ApoE, is the most important genetic risk factor for sporadic ...
After treatment, brains were collected and subjected to immunohistochemical and biochemical analyses to assess the myelination status, microglial responses, and their capacity for myelin debris clearance. Bulk RNA sequencing was performed on the corpus callosum (CC) to address the molecular mechanisms ...
Dysfunction of oligodendrocytes, the myelinating cells of the CNS, may contribute to AD pathogenesis (Nugent et al., 2020; Zhou et al., 2020). Therefore, we assessed oligodendrocyte populations in our snRNA-seq dataset. One major oligodendrocyte cluster (cluster 2) was identified from the origin...
(Cat. #130-092-628, Miltenyi Biotec), and myelin was removed using magnetic bead separation (Myelin Removal Beads II, Cat. #130-096-733, Miltenyi Biotec). After myelin removal, microglia and debris were removed by magnetic separation using anti-CD11b+ microbeads (Cat. #130-049-601, ...
(L10KJ; BasalMedia, Shanghai, China) at 37 °C. After myelin was removed by the 30% Percoll gradient (CP8331; Coolaber, Beijing, China) at 500 × gfor 30 min, the total cell pellet was resuspended in DMEM and passed through a 70-μm filter. Dissociated cells were surface ...
Several genetic risk factors for Alzheimer’s disease implicate genes involved in lipid metabolism and many of these lipid genes are highly expressed in glial cells1. However, the relationship between lipid metabolism in glia and Alzheimer’s disease pat
In response, astrocytes (FIG. 1, Step 2) and macrophages synthesize and release ApoE within the lesion to scavenge cholesterol from both cellular and myelin debris. Using the activity of the 3,3-hydroxy-methylglutaryl-CoA reductase (HMG-COA reductase) to monitor cholesterol synthesis, it was ...