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After incubation, we depleted myelin debris by 30% isotonic Percoll (P1644; Sigma-Aldrich) gradient centrifugation and obtained mononuclear cell suspensions in DMEM/F12 medium with ice-cold 10% heat-inactivated fetal bovine serum. We prepared unstained controls from mixtures of different sample cell ...
We also assessed plasma protein levels of neurofilament light chain (NFL), a marker of neuroaxonal damage and neurodegeneration (Khalil et al., 2018). We observed a significant reduction in plasma NFL concentration in TAM-treated TAFE4 mice (Figure 2G). Altogether, these results implicate ...
We also use optional cookies for advertising, personalisation of content, usage analysis, and social media. By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with...
After incubation, we depleted myelin debris by 30% isotonic Percoll (P1644; Sigma-Aldrich) gradient centrifugation and obtained mononuclear cell suspensions in DMEM/F12 medium with ice-cold 10% heat-inactivated fetal bovine serum. We prepared unstained controls from mixtures of different sample cell ...
Poor lipidation of apoE4 impairs its ability to opsonize extracellular debris such as fibrillar Aβ42 and myelin breakdown products, which impairs microglial recognition and clearance responses [10,11]. Rather than clearing debris effectively, APOEɛ4 microglia show phagocytic and endolysosomal dysfuncti...